桑根皮（又称桑白皮）是一种传统中药材，具有降血压、抗病毒、抑菌和抗炎等药理活性，本课题组前期研究发现，桑根皮乙酸乙酯提取物具有较好的抗病毒和抑菌活性，为探究桑根皮药理活性的物质基础，本文研究了广东桑（Morus atropurpurea Roxb.）根皮的化学成分。运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、ODS柱色谱和制备高效液相色谱等技术，从广东桑根皮乙酸乙酯提取物中分离纯化到15个化合物，根据化合物的理化性质和波谱数据进行结构解析，将15个化合物分别鉴定为kuwanon A（1）、kuwanon B（2）、kuwanon C（3）、kuwanon T（4）、cyclomorusin（5）、moracin M（6）、moracin O（7）、moracin P（8）、mulberrofuran L（9）、albanin A（10）、australone A（11）、5'-（1'，1'-dimethylallyl）-8-（3'，3'-dimethylallyl）-2'，4'，5，7-tetrahydroxyflavone（12）、5，7-dihydroxycoumarin（13）、oxyresceratrol（14）和β-daucosterol（15）。15个化合物按照结构可分为异戊烯基黄酮类（8个）、2-芳基苯并呋喃类（4个）、香豆素类（1个）、二苯乙烯类（1个）和甾醇类（1个），其中化合物1~4，7~13均为首次从广东桑中分离得到。

Mulberry root bark (Sang Bai Pi) was a kind of traditional Chinese medicine which showed hypotensive, antiviral bacteriostatic and anti-inflammatory activities. In order to explore the material basis of pharmacological activity of mulberry root bark, the chemical constituents of the root bark from Morus atropurpurea was studied in this paper. Fifteen compounds were isolated from the root bark by silica gel, Sephadex LH-20, ODS and PHPLC column chromatography. Their structures were identified by physicochemical properties and spectral analysis as kuwanon A (1), kuwanon B (2), kuwanon C (3), kuwanon T (4), cyclomorusin (5), moracin M (6), moracin O (7), moracin P (8), mulberrofuran L (9), albanin A (10) , australone A (11), 5'-(1',1'-dimethylallyl)-8-(3',3'-dimethylallyl)-2',4',5,7-tetrahydroxyfla-vone (12), 5,7-dihydroxycoumarin (13), oxyresceratrol (14) and β-daucosterol (15), respectively. Fifteen compounds contained eight prenylflavonoids, four 2-arylbenzofuran, one coumarin, one stilbene and one sterol. Moreover, compounds 1-4 and 7-13 were firstly found from Morus atropurpurea.

港澳台科技合作专项资助（2013DFM30080）；教育部重大项目培育基金（707048）；广东省高校国际科技合作创新平台项目（gjhz1003）