对重庆市售苦丁茶进行SD大鼠胃损伤预防效果研究。在本实验中两个浓度的苦丁茶样品和阳性药物奥美拉唑被用以检测。苦丁茶和奥美拉唑处理大鼠相对于对照组大鼠降低了血液中IL-1β、IL-6和IL-8炎症细胞因子水平；升高了IL-4因子水平。苦丁茶和阳性药物奥美拉唑组的血清SS和VIP水平高于对照组，MOT和SP水平低于对照组。模型对照大鼠、500 mg/kg苦丁茶处理大鼠、1000 mg/kg苦丁茶处理大鼠、奥美拉唑处理大鼠和正常大鼠的呈胃液量由高到低排列，各组大鼠胃液pH值表现出相反的趋势。苦丁茶显著降低的大鼠胃损伤程度，表明其具有抗炎症特性，1000 mg/kg苦丁茶表现出强抗炎症效果，仅比奥美拉唑略差。通过RT-PCR分析，苦丁茶处理组大鼠胃组织的iNOS和COX-2炎症mRNA表达较模型对照组大鼠显著下降。由这些结果得出苦丁茶具有和药物对照奥美拉唑一样的胃损伤预防效果。

Commercial Ilex kudingcha C.J. Tseng (KDC) was purchased from Chongqing market for the evaluation of gastric injury preventive effect in SD-rat. Two concentrations of KDC samples and omeprazole as pharmic control were tested. KDC and omeprazole reduced the levels of serum proinflammatory cytokines of IL-1β, IL-6, IL-8, and increased the IL-4 level as compared to the control in rats. SS and VIP serum levels of KDC and omeprazole groups were higher than those of the control group, while MOT and SP serum levels of KDC and omeprazole groups were lower than those of the control group. The gastric secretion volumes from high to low order were control rats, 500 mg/kg KDC-treated rats, 1000 mg/kg KDC-treated rats, omeprazole-treated rats, and normal rats, whereas pH levels of gastric juice showed the opposite trend. The gastric injury level was significantly decreased by KDC, demonstrating its anti-inflammatory properties. 1000 mg/kg of KDC showed the strongest anti-inflammatory effect that similar to the clinical drug omeprazole. For gastric tissues of KDC groups and omeprazole group rats, RT-PCR assay showed that the mRNA expression levels of inflammation-related genes of iNOS and COX-2 were significantly decreased, as compared with the control group. These results suggested that KDC had a gastric injury preventive effect as omeprazole.

重庆高校创新团队建设计划资助项目（KJTD201325）