本文采用高效液相色谱-二极管阵列检测器法（HPLC-DAD）快速检测杏中水溶性有机酸（苹果酸，柠檬酸和琥珀酸）和维生素（维生素C、维生素B1、维生素B2）含量。以0.5 mmol/L磷酸-乙腈为流动相，随着时间推移改变流速和流动相比例，13 min内梯度洗脱，采用多检测波长210 nm，254 nm和270 nm条件下，可使所测有机酸和维生素实现快速较好地分离。方法的稳定性在0.75~2.12%范围内，精密度在0.94~1.55%之间，回收率测定结果为96.7~101.9%。采用本方法测定不同地域杏样品，提出巨鹿杏的特征性品质。

High performance liquid chromatography (HPLC) with diode array detector (DAD) was applied to determine water-soluble organic acids (malic acid, citric acid and succinic acid) and vitamins (Vc, VB1 and VB2) in apricots sampled from Julu. The apricots samples were eluted using 0.5 mmol/L H3PO4 -acetonitrile as mobile phase at several wavelengths (210 nm, 254 nm and 270 nm) . The substances can be efficiently separated in 13 min with RSD value ranged in 0.75~2.12%. The precisions were between 0.94% and 1.55% and the result of recoveries was of 96.70~101.9%. The difference in organic acid and vitamin contents between Julu apricot and those sampled from other sources were also compared.

河北省科学技术研究与发展计划项目（12221003D）