为了探索罗非鱼皮胶原蛋白抗氧化肽的最佳脱盐工艺，本文采用DA201-C大孔吸附树脂对抗氧化肽进行脱盐试验。结果表明：DA201-C大孔吸附树脂对抗氧化肽的静态吸附率和吸附量分别为82.56%和54 mg/g；动态吸附和解吸最佳的上样浓度为60 mg/mL，最佳上样流速为1 mL/min，解吸剂为75%乙醇。抗氧化肽经过DA201-C大孔吸附树脂处理后脱盐率为97.13%，回收率为81.16%，质量浓度为0.245 mg/mL时对ABTS自由基的清除率提高至39.58%。采用DA201-C大孔吸附树脂对罗非鱼皮胶原蛋白抗氧化肽进行脱盐处理是一种简便有效的方法。

The desalting technology of antioxidant peptides from tilapia skin collagen by DA201-C macroporous adsorption resin was investigated. The results showed that the static absorption rate and absorbing capacity were 82.56% and 54mg/g, respectively. The best loading sample concentration, sampling flow rate and eluent were 60 mg/mL, 1 mL/min and 75% alcohol, respectively. Under these conditions, the desalination rate was 97.13% and recovery of peptides was 81.16%. The antioxidant activity was increased to 39.58% at the concentration of 0.245 mg/mL. In conclusion, it was available and effective to desalt the antioxidant peptides from tilapia skin collagen by using DA201-C macroporous absorption resin.

“十二五”农村领域国家科技计划课题(2012BAD28B06)；海口市重点科技计划项目(2011-079)