[关键词]
[摘要]
优化加拿大原产地西洋参皂苷的最佳提取工艺。采用HPLC-ELSD法,在单因素试验基础上,利用响应面法,以人参皂苷Rb1为响应值,建立数学模型,对提取温度、时间、乙醇浓度和料液比进行优化,确定最佳提取工艺。结果表明:提取温度为90 ℃、乙醇浓度77.4%、提取时间1.88 h、料液比1:25、提取2次的人参皂苷Rb1提取率可达到1.40%,与预测值1.41%相差不大。
[Key word]
[Abstract]
The Response Surface Methodology (RSM) was used to optimize the extraction process of ginsenoside from P. quinquefolius L. collected from Canada. Based on one-factor-at-a-time experiments, extract yield of the ginsenoside-Rb1 was used as response for establishing a quadratic regression model. Temperature and time of extraction, ethanol concentration and liquid-to-solid ratio were selected as the factors for RSM design. The results showed that the best extraction conditions were as follows: extraction temperature 90 ℃, extraction time 1.88 h, ethanol concentration 77.4%, solid-liquid ratio 1:25, and extracted times twice, under which the predicted yield of ginsenoside-Rb1 with the optimized procedure was up to 1.40%and practical yield was 1.41%.
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[基金项目]
国家科技部“重大新药创制”(2011ZX09307-002-01);国家科技支撑计划(2012BAI27B07);国家自然基金(81173653);四川省杰出青年学术技术带头人后续计划(2011JQ0051)