预测耐热β-半乳糖苷酶的初级结构和高级结构，为进一步了解该蛋白的结构特征提供理论依据。应用ProtParam、ProtScale、TMHMM Server v. 2.0、SignalP 4.0 Server、SOPMA、PSORT Prediction、GENO3D等生物信息学软件对此耐热β-半乳糖苷酶的初级结构和空间结构进行分析和预测。该耐热β-半乳糖苷酶由452个氨基酸残基组成，无跨膜区域和信号肽，定位于细胞质，二级结构以α螺旋和无规则卷曲为主，应用同源建模的方法，三维结构分子模型得到了成功构建。为进一步研究耐热β-半乳糖苷酶的功能特征以及改造奠定了基础。

This study aimed to predict the primary and higher structures of thermostable β-galactosidase, providing theoretical basis for further study on its structural characteristics. Several kinds of bioinformatics softwares, including ProtParam, ProtScale, TMHMM Server v. 2.0, SignalP 4.0 Server, SOPMA, PSORT Prediction and GENO3D, were used to analyse and predict the primary and spatial structure of this enzyme. Result showed that the thermostable β-galactosidase was composed of 452 amino acids residuals. The enzyme protein had no signal peptide and transmembrane region, and located in the cytoplasm. Alpha Helix and Random coil were main structures in its secondary structure. By using homology modeling, three-dimensional structure of the enzyme was constructed.

“十二五”农村领域国家科技计划课题(2012BAD28B0903)