对源自Bacillus subtilis 168的具有纤溶活性的基因序列（WprA）进行克隆，然后将WprA基因克隆到大肠杆菌-枯草杆菌穿梭载体 pBE3中，构建表达载体pBE-WprA，将重组载体转化到Bacillus subtilis WB800中，实现了WprA基因在Bacillus subtilis WB800中的表达。结果表明，WprA基因在Bacillus subtilis WB800中的对数生长期和平衡期均获得了表达，且产物被分泌到胞外。

A fibrinolytic enzyme gene (WprA) was cloned from Bacillus subtilis 168. To efficiently express WprA in Bacillus subtilis WB800, WprA gene was inserted into pBE3 to yield a nove vector pBE-WprA. Then the vector pBE-WprA was transformed and expressed in Bacillus subtilis WB800. Results showed WprA gene was efficiently expressed during the exponential and stationary growth stages, and WprA was secreted into the medium.

中央高校基本科研业务费项目（2011ZM0136）