以罗非鱼内脏为原料，研究内源蛋白酶酶学特性；利用正交试验对其自溶工艺进行优化，结果表明：罗非鱼内脏内源蛋白酶在pH 5~11范围内有较好的稳定性，在40~50 ℃条件下，罗非鱼内脏蛋白酶活性最稳定；罗非鱼内脏自溶的最佳工艺为：pH 8，温度45 ℃，料液比1:2条件下的自溶4 h产物氮收率为73.21%，水解度为18.78%。

The enzyme characteristics of endogenous protease were analyzed and the optimum autolysis conditions were determined by the orthogonal test. Result showed that the endogenous protease of Tilapia viscera was stable at pH of 5~11 and 40~50℃. The optimum conditions for autolysis of viscera were pH 8, temperature 45℃, raw/water materials 1:2 and reaction time 4 h, under which the nitrogen recovery ratio and degree of hydrolysis were 73.21% and 18.78% respectively.

广东省科技计划项目（2009B020313003）；广东省重大科技专项（2010A080403002）