白藜芦醇对过氧化氢诱导的间充质干细胞氧化损伤的保护作用

Protective Effect of Resveratrol on Hydrogen Peroxide-Induced Oxidative Damage in Mesenchymal Stem Cells

摘要:

为探究白藜芦醇对过氧化氢（Hydrogen Peroxide, H2O2）诱导的间充质干细胞氧化损伤的保护作用及可能机制。本研究采用CCK-8法测定H2O2处理间充质干细胞24 h的半抑制浓度（IC50），并以此建立氧化损伤模型。实验分为正常组、模型组（500 μmol·L-1 H2O2）、阳性药组（维生素C）以及白藜芦醇低（25 μmol·L-1）、中（50 μmol·L-1）、高（100 μmol·L-1）剂量组。结果表明，与模型组相比，白藜芦醇中、高剂量预处理效果更为明显，间充质干细胞的相对凋亡率和相对活性氧水平均显著降低，相对凋亡率分别为4.66和3.01，相对活性氧水平分别为6.23和4.39（正常组为参照，其值为1）。此外，超氧化物歧化酶（SOD）和谷胱甘肽（GSH）含量升高，丙二醛（MDA）含量降低；抗氧化相关基因（Nrf2、NQO1、HO-1）的表达水平上调。综上所述，白藜芦醇预处理能有效减轻H2O2诱导的间充质干细胞氧化损伤，其机制可能与其激活Nrf2信号通路以及增强内源性抗氧化能力存在紧密关联。该研究为白藜芦醇在提升间充质干细胞移植存活率和治疗效率方面提供了理论基础。

Abstract:

The protective effects and possible mechanisms of resveratrol on hydrogen peroxide (H2O2)-induced oxidative injury in mesenchymal stem cells were investigated. In this study, the half inhibitory concentration (IC50) of mesenchymal stem cells treated with H2O2 for 24 h was determined by the CCK-8 method, and an oxidative injury model was established based on this concentration. The experiment was divided into a normal group, a model group (500 μmol·L-1 H2O2), a positive control group (vitamin C), and resveratrol low- (25 μmol·L-1), medium- (50 μmol·L-1), and high-dose (100 μmol·L-1) groups. The results showed that compared with the model group, a more pronounced effect was observed with medium- and high-dose resveratrol pretreatment, as both the relative apoptosis rate and relative reactive oxygen species levels of mesenchymal stem cells were significantly reduced, with relative apoptosis rates of 4.66 and 3.01, and relative reactive oxygen species levels of 6.23 and 4.39, respectively (using the normal group as the reference, with its value set as 1). In addition, the contents of superoxide dismutase (SOD) and glutathione (GSH) were increased, and the content of malondialdehyde (MDA) was decreased; the expression levels of antioxidant-related genes (Nrf2, NQO1, and HO-1) were upregulated. In conclusion, H2O2-induced oxidative injury in mesenchymal stem cells was effectively alleviated by resveratrol pretreatment, and the mechanism may be closely associated with the activation of the Nrf2 signaling pathway and the enhancement of endogenous antioxidant capacity. This study provides a theoretical basis for resveratrol in improving the survival rate and therapeutic efficiency of mesenchymal stem cell transplantation.