Abstract:Crispness is recognized as an important quality attribute influencing the processing suitability and storage quality of peppers. To address the low accuracy associated with traditional crispness measurement methods, an accurate and rapid puncture-based method for determining the crispness of fresh peppers was established using a texture analyzer in this study. Four pepper varieties (A, B, C, and D) were selected as test materials, and the puncture method was compared with single-compression and multi-compression methods. The accuracy of the measurement approach was further verified through sensory evaluation and microscopic analysis of cellular structures. The results demonstrated that, compared with the single-compression and multi-compression methods, the puncture method exhibited a high degree of curve overlap and a coefficient of variation (CV) of less than 10%, and significant differences in crispness among the four pepper varieties were detected (P<0.05). The crispness ranking determined based on differences in cellular structure was A>C>B>D; the sensory evaluation rank sums were 43, 25, 34, and 18, respectively, which were consistent with the crispness results obtained by the puncture method. During the storage period of 0~12 days, the puncture method was found to accurately reflect the changing trend of pepper crispness, with smaller measurement errors than other methods. In conclusion, the puncture method using a texture analyzer is suitable for measuring the crispness of fresh peppers. The method provides high measurement accuracy and offers scientific support and technical guidance for the standardized detection, evaluation, and utilization of pepper crispness.