粗毛纤孔菌活性物质提取及其黄嘌呤氧化酶抑制作用

Extraction of Active Substances from Inonotus hispidus and Inhibitory Effects on Xanthine Oxidase

摘要:

为了实现粗毛纤孔菌（Inonotus hispidus）的高效利用和开发，该研究在单因素实验的基础上，采用响应面法优化超声波辅助提取粗毛纤孔菌活性物质醇提物（Inonotus hispidus ethanol extract，IHE），依次制备粗毛纤孔菌水提物（Inonotus hispidus water extract，IHW）和酶解物（Inonotus hispidus enzymatic hydrolysate，IHEH），并对粗毛纤孔菌三种提取物进行成分分析。此外，为评价粗毛纤孔菌三种不同提取物对黄嘌呤氧化酶（Xanthine Oxidase，XOD）的抑制作用，进一步探究了其对XOD活性的抑制效果。结果发现，超声波辅助提取IHE的最佳工艺为超声温度53℃，超声时间1.7 h，料液比1:53 g/mL，在此条件下IHE的得率为27.14%。黄嘌呤氧化酶活性抑制实验结果显示，粗毛纤孔菌三种不同提取物均具有显著的抑制XOD活性效果，且在一定质量浓度范围下呈剂量依赖性，IHE、IHW、IHEH的EC50值分别为2.19 mg/mL、3.18 mg/mL、2.97 mg/mL，由此可知IHE抑制效果最好。综上，粗毛纤孔菌活性物质不同提取物均具有潜在的降尿酸活性，为粗毛纤孔菌降尿酸功能性食品的开发提供了理论依据。

Abstract:

In order to achieve efficient utilization and development of Inonotus hispidus, this study optimized the ultrasonic-assisted extraction of the ethanol extract of Inonotus hispidus (IHE) based on single-factor experiments using response surface methodology. Subsequently, the water extract (IHW) and enzymatic hydrolysate (IHEH) of Inonotus hispidus were prepared in sequence, and the components of the three extracts of Inonotus hispidus were analyzed. In addition, to evaluate the inhibitory effects of the three different extracts of Inonotus hispidus on xanthine oxidase (XOD), the inhibitory effects on XOD activity were further investigated. The results show that the optimal process for ultrasonic-assisted extraction of IHE is an ultrasonic temperature of 53℃, ultrasonic time of 1.7 h, and solid-liquid ratio of 1:53 g/mL. Under these conditions, the yield of IHE is 27.14%. The results of the xanthine oxidase (XOD) activity inhibition experiment show that the three different extracts of Inonotus hispidus all have significant inhibitory effects on XOD activity, and the inhibitory effects are dose-dependent within a certain concentration range. The predicted EC50 values of IHE, IHW, and IHEH are 2.19 mg/mL, 3.18 mg/mL, and 2.97 mg/mL, respectively.Therefore, it can be concluded that IHE has the best inhibitory effect. In conclusion, the different extracts of active substances from Inonotus hispidus all have potential uric acid-lowering activity, which provides a theoretical basis for the development of uric acid-lowering functional foods from Inonotus hispidus.