Abstract:Abstract: In order to investigate the effects of the synergistic treatment of low temperature (0 ± 1°C), ozone (O3) fumigation and gas-conditioned packaging(MAP) on the postharvest quality of fresh American ginseng, the experiment was conducted using 0.5 × 10-6 O3 fumigation for 30min in combination with four gas-conditioned packaging materials (IE3900-MY, IE3900-L400, IE3900-MK10 and L400 PBAT-HP101), which PE group was used as the control. The preservation quality was comprehensively evaluated by multi-dimensional indexes (appearance quality, CO2 and O2 content in the bag, water loss rate, spoilage rate, antioxidant enzyme activity, saponin content and glycoside content). The results showed that the treatment with O3 fumigation and L400 PBAT-HP101 packaging led to significantly delay on the quality deterioration. on the 120th day of storage, the water loss rate decreased by 1.19% and the decay rate decreased by 7 times. The treatment with O3 fumigation and IE3900-MK10 packaging resulted in the antioxidant defense system and the activities of antioxidant enzymes were activated, such as PAL, SOD and CAT, were increased by 13.39%, 5.19% and 11.16%, respectively. Additionally, the treatment with O3 fumigation with IE3900-MY packaging and the treatment with IE3900-L400 packaging regulated CO2 and O2 levels, reducing CO2 content by approximately 23.85% and increasing O2 content by 13.24% compared to the PE group. Furthermore, the treatment with O3 fumigation with L400 PBAT-HP101 packaging was shown to regulate the saponin metabolism: after 120 d of storage, although the Rg1 content was reduced by 28.24%, the Re and Rb1 contents were increased by 23.44% and 52.46%, respectively. In conclusion, the treatment with O3-MAP synergistic technology was demonstrated to prolong the storage period by regulating enzyme activity and saponin homeostasis, and the treatment with O3 fumigation and L400 PBAT-HP101 was identified as the the most effective in preserving the intrinsic quality and saponin compositions of fresh American ginseng. The results of this study can provide a theoretical basis and technical support for the green preservation of high value-added traditional Chinese medicinal materials.