即食红参片对秀丽隐杆线虫抗疲劳作用

Anti-Fatigue Effect of Ready-To-Eat Red Ginseng Tablets on Caenorhabditis Elegans

摘要:

为了探讨即食红参片的抗疲劳功效，该研究通过测定即食红参片的主要活性成分，再以秀丽隐杆线虫(Caenorhabditis elegans)为模型，测定其寿命、运动能力、吞咽频率、体内抗氧化能力及抗疲劳相关基因表达量等指标，评价即食红参片（JSHS）、干红参饮片(HSYP)及组方液（ZFY）的抗疲劳效果。结果表明，JSHS中的总皂苷、总多糖含量高于HSYP与ZFY；线虫实验中，与空白组对比，JSHS、HSYP分别使线虫的平均寿命提高了16.56%、10.85%；JSHS使线虫的吞咽频率极显著提高了25.32%(P<0.0001)；JSHS使线虫抗应激能力显著提高(P<0.01)，并使其体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性均显著提高(P<0.05)，丙二醛(MDA)的含量显著下降(P<0.05)；JSHS极显著下调daf-2基因与上调daf-16、sod-3、skn-1基因的表达量（P<0.0001）。综上，经HSYP配伍ZFY加工而成的JSHS，其主要活性成分含量得到显著提高，且JSHS通过延长线虫寿命、提高其运动能力与吞咽频率、增强抗应激能力及体内抗氧化能力，并调节抗疲劳相关基因的表达，综合增强线虫的抗疲劳能力。该研究初步表明即食红参片具有抗疲劳功效，为保健食品开发应用提供理论基础。

Abstract:

To explore the anti-fatigue effect of ready-to-eat red ginseng tablets, the main active components of ready-to-eat red ginseng tablets were determined in this study. Then, using Caenorhabditis elegans as the model, indicators such as lifespan, locomotor ability, pharyngeal pumping rate, in vivo antioxidant capacity, and expression levels of anti-fatigue-related genes were measured to evaluate the anti-fatigue effects of ready-to-eat red ginseng tablets (JSHS), dried red ginseng decoction pieces (HSYP), and formula solution (ZFY).The results showed that the contents of total saponins and total polysaccharides in JSHS were higher than those in HSYP and ZFY. In the Caenorhabditis elegans experiment, compared with the blank group, JSHS and HSYP increased the average lifespan of Caenorhabditis elegans by 16.56% and 10.85%, respectively. The pharyngeal pumping rate of Caenorhabditis elegans was extremely significantly increased by 25.32% (P<0.0001) by JSHS. The stress resistance of Caenorhabditis elegans was significantly improved by JSHS (P<0.01), and the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in Caenorhabditis elegans were all significantly increased (P<0.05), while the content of malondialdehyde (MDA) was significantly decreased (P<0.05) by JSHS. The expression level of the daf-2 gene was extremely significantly down-regulated, and the expression levels of the daf-16, sod-3, and skn-1 genes were extremely significantly up-regulated in Caenorhabditis elegans by JSHS (P<0.0001). In conclusion, the content of main active components in JSHS, which is processed by combining HSYP with ZFY, was significantly increased. Moreover, JSHS comprehensively enhanced the anti-fatigue ability of Caenorhabditis elegans by extending their lifespan, improving their locomotor ability and pharyngeal pumping rate, enhancing their stress resistance and in vivo antioxidant capacity, and regulating the expression of anti-fatigue-related genes. This study preliminarily indicates that ready-to-eat red ginseng tablets have anti-fatigue effects, providing a theoretical basis for the development and application of health food.