基于OPLS-DA分析五省甜瓜矿物元素含量特征及RNI膳食评价

Contents and RNI Values of Mineral Elements in Melons Harvested in Five Provinces Characterized via OPLS-DA Analysis

摘要:

以5省份128份甜瓜果实样品为研究对象，参照GB 5009.268-2016，通过电感耦合等离子体质谱仪（ICP-MS）开展了样品中13种矿物元素含量的定量分析，进而综合多元统计分析，包括正交偏最小二乘法判别分析（OPLS-DA）、相关性分析、推荐摄入量（RNI）贡献率等方法，筛选不同省份甜瓜特征矿物元素，参照中国营养学会制定的相关元素RNI值，对不同人群进行元素营养水平分析与评价。结果表明：B、Fe、Sr、Mn、Zn为5省份的差异元素（VIP>1），B元素为差异最大的元素（VIP>2，组间差异P<0.001），B、Fe、Sr、Mn、Zn元素水平最高的省份分别为：江苏（18.569 mg/kg）、海南（8.178 mg/kg）、内蒙古（5.098 mg/kg）、海南（2.302 mg/kg）、海南（3.662 mg/kg）；广西、海南、河北、江苏、内蒙古甜瓜的RNI 贡献优势元素分别为Sn、Mn、Mo、Zn、Fe。对3种人群Mn、Zn、Fe、Sn的RNI贡献率较高的分别是是海南省和广西省。研究为5省甜瓜地域优势元素发展提供了参考，依据不同人群饮食需求，给出5省份甜瓜的饮食建议，可支撑和推动区域甜瓜的品牌创建与提质增效。

Abstract:

A total of 128 melon samples harvested in five provinces were quantitatively analyzed for 13 mineral elements using an inductively coupled plasma mass spectrometer, following the GB 5009.268-2016 standard. Next, the characteristic mineral elements in samples from each province were identified using orthogonal partial least squaresdiscriminant analysis (OPLS-DA), correlation analysis, and percentage contribution to recommended nutrient intake (RNI). With reference to the RNI values of corresponding elements specified by the Chinese Nutrition Society, the mineral nutrient levels in different populations were analyzed and evaluated. The results show that the levels of B, Fe, Sr, Mn, and Zn differed most among the five provinces (VIP>1), with the difference in B being the most prominent (VIP>2, P<0.001 for betweengroup differences). The highest levels of B, Fe, Sr, Mn, and Zn were identified in Jiangsu (18.569 mg/kg), Hainan (8.178 mg/kg), Inner Mongolia (5.098 mg/kg), Hainan (2.302 mg/kg), and Hainan (3.662 mg/kg), respectively. The elements with the highest percentage contribution to RNI in Guangxi, Hainan, Hebei, Jiangsu, and Inner Mongolia were Sn, Mn, Mo, Zn, and Fe, respectively. Furthermore, Mn, Zn, Fe, and Sn had the highest percentage contribution to RNI in children, male adult, and female adults in Hainan and Guangxi. Overall, these findings serve as a reference for identifying region-specific dominant mineral elements in melons from the five provinces. Dietary recommendations about melons from the five provinces are provided according to the dietary needs of different groups, aiming to support regional brand development and enhance the quality and efficiency of melon production.