低温等离子体激发介质对罗非鱼片脂质氧化和酶活性影响

Effects of Cold Plasma Excitation Media on Lipid Oxidation and Enzyme Activity of Tilapia Fillets

摘要:

该研究通过脂质初级和次级氧化产物含量变化，脂肪氧合酶（Lipoxygenase, LOX）、脂肪酶、酸性脂肪酶、中性脂肪酶和磷脂酶活性变化，评价了激发介质（GasA：10% O2:50% N2:40% CO2，GasB：22% O2:78% N2，GasC：30% O2:30% N2:40% CO2）对低温等离子体处理罗非鱼片冷藏过程中脂质氧化和酶活性影响。结果表明：低温等离子体处理能够促进罗非鱼片脂质氧化，同时降低脂质氧化相关酶活性。在冷藏期间，10% O2含量处理组中罗非鱼片过氧化值和硫代巴比妥酸值为0.49 meq/kg和1.05 mg/kg，显著低于21%和30% O2处理组（P <0.05）。LOX、脂肪酶、酸性脂肪酶、中性脂肪酶和磷脂酶的酶活性在冷藏期间均呈现先上升后下降的变化趋势，并在第2天时达到最大。上述5种酶失活程度随处理组中O2含量的升高而增加，在30% O2含量时酶活性最低，10% O2含量时酶活性最高。因此，低温等离子体激发介质中低O2含量，有利于延缓罗非鱼片脂质氧化，并维持的氧化相关酶活性。该研究结果为调控低温等离子体处理对罗非鱼脂质氧化提供理论基础和技术支撑。

Abstract:

In this paper, the effects of excitation media (GasA: 10% O2: 50% N2: 40% CO2, GasB: 22% O2: 78% N2, GasC: 30% O2: 30% N2: 40% CO2) on lipid oxidation and enzyme activity of tilapia fillets treated with cold plasma during cold storage were evaluated based on the changes in lipid primary and secondary oxidation products and the activities of lipoxygenase (LOX), lipase, acid lipase, neutral lipase, and phospholipase. The results showed that cold plasma treatment could promote lipid oxidation in tilapia fillets and reduce the activities of lipid oxidation-related. During cold storage, the peroxide value and thiobarbituric acid value of tilapia fillets in the 10% O2 treatment group were 0.49 meq/kg and 1.05 mg/kg, which were significantly lower than those of the 21% and 30% O2 treatment groups (P<0.05). The enzyme activities of LOX, lipase, acid lipase, neutral lipase, and phospholipase all showed a trend of iniital increase then decrease during cold storage, reaching their maximum on the second day. The degree of inactivation of the above five enzymes increased with the increase in O2 content in the treatment group, with the enzyme activity being the lowest at a 30% O2 content and the highest at a 10% O2 content. Therefore, a low O2 content in cold plasma excitation medium is conducive to delaying lipid oxidation in tilapia fillets and maintaining the activities of oxidation-related enzymes. The results of this study provide a theoretical basis and technical support for regulating the lipid oxidation of tilapia by cold plasma treatment.