普洱茶茶褐素对脂多糖诱导的大鼠肝脏损伤的保护作用

Pu-erh Tea Theabrownin’s Protection for Lipopolysaccharide - caused Liver Injury in Rats

摘要:

为了探究普洱茶茶褐素对脂多糖（LPS）诱导的大鼠肝脏损伤的保护作用。将雄性SD大鼠随机分成正常组（Control）、LPS模型组、茶褐素低、中、高剂量组（即给药量分别为0.135、0.405、1.215 g·kg-1 bw），每三天注射一次LPS（1.5 mg·kg-1）建立大鼠肝脏损伤模型，并进行为期21d的干预实验。期间记录大鼠的生长状况，实验结束后计算大鼠的肝脏指数并检测肝脏组织中谷丙转氨酶（ALT）、谷草转氨酶（AST）活性，通过HE染色观察肝组织病理变化。检测血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）活性、TC（总胆固醇）、TG（总甘油三酯）、HDL-C（高密度脂蛋白）、LDL-C（低密度脂蛋白）含量。结果显示，与Control组相比，LPS组大鼠肝细胞排列紊乱、伴有大量炎性细胞浸润及少量肝细胞坏死，脾脏脾小结紊乱及红白髓界限模糊，脾脏指数、肝脏指数、血清中ALT活性和TG含量显著升高（P<0.05），而高剂量的普洱茶茶褐素能够显著降低大鼠脾脏和肝脏指数、血清中ALT活性和TG含量（P<0.05），降幅分别为13.69%、41.10%、41.34%%和37.50%，且能缓解LPS诱导的大鼠肝脏和脾脏组织病理损伤。结果表明，普洱茶茶褐素对LPS诱导的大鼠肝脏损伤有一定的保护作用，为普洱茶茶褐素的开发利用提供理论依据。

Abstract:

The protective effect of Pu-erh Tea Theabrownin on liver injury induced by lipopolysaccharide (LPS) in rats was explored. SD rats were randomly allocated into two experimental groups, namely the normal control group and the lipopolysaccharide (LPS)-induced model group, Pu-erh tea theabrownin group low-dose, medium-dose and high-dose groups (0.135, 0.405 and 1.215 g·kg-1 bw). The liver injury model of rats was established by injecting LPS (1.5 mg·kg-1) every three days, and the intervention experiment lasting for 21 days was conducted. At the end of the animal experiment, the liver index was initially computed. Subsequently, the enzymatic activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver tissues were measured. Moreover, histopathological alterations in liver tissues were scrutinized through hematoxylin-eosin (HE) staining. Additionally, the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), as well as the contents of total cholesterol (TC), total triglyceride (TG), high - density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in serum, were detected. Results showed that compared with the Control group, the LPS group exhibited disordered arrangement of hepatocytes accompanied by extensive inflammatory cell infiltration and focal hepatocyte necrosis, accompanied by disorganized splenic nodules and indistinct red/white pulp boundaries in the spleen. Furthermore, significant elevations were observed in spleen index, liver index, serum ALT activity, and TG content (P<0.05). while the high dose of Pu-erh tea theabrownins could significantly reduce the liver index, serum ALP activity, and serum TG content in rats (P<0.05). The reductions were 24.8%, 41.3%, 34.1%, and 37.5%, respectively. Moreover, the histopathological damage caused by LPS to the liver and spleen tissues of rats was alleviated. The results showed that the liver injury induced by LPS in rats was protected to a certain extent by the Pu-erh Tea Theabrownin, providing a theoretical basis for the development and utilization of Pu-erh Tea Theabrownin.