紫灵芝多糖改善运动疲劳小鼠学习记忆能力

Ganoderma sinense Polysaccharide Improves Learning and Memory Abilities of Mice with Exercise-induced Fatigue

摘要:

该研究探究紫灵芝多糖对运动疲劳小鼠学习记忆能力的改善作用。采用水提醇沉得到紫灵芝多糖（Ganoderma sinense polysaccharide, GSP），分析其单糖组成并测定小鼠相关生化指标。结果表明，GSP经分离纯化得到3个多糖组分（GSP-A1、GSP-B1、GSP-C1），GSP-A1由Fuc、Rib、Man、Gal、Glu、Ara、Rha组成，GSP-B1由Man、Fru、Gal、Glu、Xyl、Glu-Ac、Gal-Ac组成，GSP-C1由Glu、Rha、Xyl、Glu-A、Gal-Ac组成。与运动疲劳模型组相比，GSP-A1、GSP-B1、GSP-C1组小鼠逃避潜伏期分别显著降低了23.82%、49.82%、39.35%，避暗潜伏期分别显著提高了29.40%、89.00%、56.49%；小鼠体内肝糖原和肌糖原含量均显著提高，海马组织内氧化应激和炎症因子水平显著降低，神经递质NE、5-HT、DA均显著提高。同时，抗凋亡基因Bcl-2 mRNA表达水平显著提高，促凋亡基因Bax、Caspase-3 mRNA表达水平显著下降。综上，GSP可通过降低氧化应激和炎症因子水平及抑制神经细胞凋亡来改善运动疲劳小鼠的学习记忆能力，为紫灵芝多糖在功能食品领域上的应用开发提供了新思路。

Abstract:

The improvement effects of Ganoderma sinense polysaccharide (GSP) on the learning and memory abilities of mice with exercise-induced fatigue were investigated in this study. GSP was obtained through water extraction and ethanol precipitation. Its monosaccharide composition was analyzed, and relevant biochemical indicators of mice were measured. The results showed that three polysaccharide components (GSP-A1, GSP-B1, GSP-C1) were obtained through the isolation and purification of GSP. GSP-A1 was composed of Fuc, Rib, Man, Gal, Glc, Ara and Rha, GSP-B1 was composed of Man, Fru, Gal, Glc, Xyl, Glu-Ac, Gal-Ac, and GSP-C1 was composed of Glc, Rha, Xyl, Glu-A, Gal-Ac. Compared with the exercise induced fatigue model group, the mice in the GSP-A1, GSP-B1, and GSP-C1 groups had significantly reduced escape latencies (by 23.82%, 49.82%, and 39.35%, respectively), significantly increased dark avoidance latencies (by 29.40%, 89.00%, and 56.49%, respectively), significantly increased levels of hepatic glycogen and muscle glycogen, significantly decreased levels of oxidative stress and inflammatory factors in the hippocampus, significantly increased levels of neurotransmitters NE, 5-HT and DA, significantly increased expression of anti-apoptosis gene Bcl-2 mRNA, and significantly decreased expression of pro-apoptosis genes Bax and Caspase-3 mRNA. In summary, GSP can improve the learning and memory abilities of mice with exercise-induced fatigue by reducing the levels of oxidative stress and inflammatory factors, while inhibiting neuronal apoptosis, which provides a new idea for the application and development of Ganoderma sinense polysaccharides in the field of functional foods.