东北地区大豆核盘菌检测方法发展现状

Current Situation of Soybean Sclerotinia Sclerotiniorum in Northeast China

摘要:

大豆菌核病分布广泛，是世界范围内的大豆病害类型。我国东北地区大豆菌核病的发病率高达60%以上，严重影响大豆产量和经济效益。故此，研究大豆菌核病致病菌——核盘菌的致病原理以及有效检测、鉴定方法现已成为我国大豆产业中亟待解决的问题。该文着重从分子层面阐述了核盘菌的致病原理，综述了现有的核盘菌检测方法：传统检测方法、血清学检测方法、分子生物学检测方法等研究进展。详述了现有检测方法的敏感性、特异性、适用性等特性，比较评价了各种方法的优势与局限，同时对大豆核盘菌的检测模型进行了初探。该研究旨在为大豆菌核病的鉴定、检测工作提供参考，为菌核病的早期发现提供技术支撑，为大豆产业的良性发展提供一些理论依据。

Abstract:

Soybean sclerotinia is widely distributed and is a type of soybean disease found worldwide. In the Northeast region of China, the incidence rate of soybean sclerotinia disease can reach over 60%, severely affecting soybean yield and economic benefits. Therefore, studying the pathogenic principle of the soybean sclerotinia disease pathogen—Sclerotinia sclerotiorum, as well as effective detection and identification methods, has become an urgent issue in China"s soybean industry. This article reviews the pathogenic mechanism of Sclerotinia sclerotiorum at the molecular level and reviews. It reviews the existing detection methods for the fungus: traditional detection methods, serological detection methods, and molecular biological [1]detection methods, among others. It details the sensitivity, specificity, and applicability of the existing detection methods, compares and evaluates the advantages and limitations of various methods, and provides an initial exploration into the detection model for soybean sclerotinia. This study aims to provide a reference for the identification and detection of soybean sclerotinia disease, to offer technical support for the early detection of the disease, and to provide some theoretical basis for the healthy development of the soybean industry.