该研究以板栗壳为研究对象，旨在探索一种绿色、高效的板栗壳黄酮物质的提取方法。首先考察不同的单因素对板栗壳总黄酮的提取效果，再通过PB实验确定显著性影响因素，板栗壳中黄酮类物质的提取工艺通过响应面实验优化得出，并在此基础上建立了板栗壳中黄酮类物质提取的动力学模型。结果表明，采用低共熔溶剂为氯化胆碱: 乙醇（摩尔比1:1）作为提取剂，板栗壳中黄酮类物质的最优提取工艺为：超声温度为40 ℃，超声功率为150 W，料液比为1:25（g/mL）、超声时间60 min，验证得出理论总黄酮得率与实际总黄酮得率的相对误差为1.49%，表明条件优化合理。该条件下的试验数据的线性回归R2 的范围为0.917 22~0.998 4，线性拟合较好，表明提取过程符合一级动力学模型。研究结果为超声辅助低共熔溶剂技术提取板栗壳中黄酮类物质提供了一定的理论支撑，并为板栗资源的充分开发利用提供了借鉴。

A green and efficient method to extract flavonoids from chestnut shells was examined. First, the effects of various single factors on the extraction of total flavonoids from chestnut shells were investigated, and significant influencing factors were identified using the Plackett-Burman experimental design. The conditions for flavonoid extraction were then optimized using response surface methodology, and, based on these optimizations, a kinetic model of the extraction process was established. The results showed that when a deep eutectic solvent composed of choline chloride and ethanol (molar ratio 1:1) was used, the optimal conditions for flavonoid extraction were as follows: an ultrasonic temperature of 40 ℃, an ultrasonic power of 150 W, a solid-to-liquid ratio of 1:25 (g/mL), and an ultrasonic time of 60 min. The relative error between the theoretical and actual total flavonoid yields was 1.49%, indicating the reasonableness of the optimized conditions. The linear regression R2 values for the experimental data under these conditions ranged from 0.917 22 to 0.998 4, demonstrating good linear fitting and suggesting that the extraction process conforms to a first-order kinetic model. These findings provide theoretical support for flavonoid extraction from chestnut shells using ultrasound-assisted extraction with deep eutectic solvents and offer a reference for the comprehensive development and utilization of chestnut resources.

河南省科技攻关项目（232102230071）；河南省高等学校重点科研项目（23B550006；24B180016；24B550016）；信阳农林学院高水平科研孵化器建设基金项目（FCL202110）；信阳农林学院青年骨干教师培养项目（2022年度）；信阳农林学院青年教师科研基金项目（QN2022030）