Abstract:A sensitive and efficient method was established and validated for the qualitative and quantitative analyses of non-volatile components of five varieties of Rosa rugosa using ultra-high-performance liquid chromatography (UPLC)-QExactive Orbitrap-mass spectrometry (MS) coupled with UPLC-triple quadropole-MS/MS methods. A Thermo Hypersil Gold chromatographic column (100 mm×2.1 mm, 3 μm) with formic acid water-formic acid acetonitrile as the mobile phase and an Agilent Poroshell 120 EC-C18 chromatographic column (4.6 mm×100 mm, 2.7 μm) with methanol-water as the mobile phase were used for gradient elution. Both positive and negative ion modes were used. The structures of 73 compounds were identified and their fragmentation patterns were analyzed using high-resolution mass spectrometry. The linear relationship of the seven flavonoid components in R. rugosa was good in concentration range at which they were observed (R>0.990), and the average recovery rate was 96.62%~107.13%, with the relative standard deviation ranging from 1.06% to 1.87%. The contents of the seven flavonoids were significantly different between the five varieties of R. rugosa. This method is rapid, reliable, and efficient, and can be used to comprehensively reflect and compare the non-volatile components and seven main flavonoid contents of five varieties of R. rugosa, which can provide reference for further elucidating the material basis and quality standard of R. rugosa.
