采用UPLC-Q-Exactive-Orbitrap-MS联合UPLC-QQQ-MS/MS对5种玫瑰花中非发挥性成分进行定性定量分析。分别采用Thermo Hypersil GOLD色谱柱（100 mm×2.1 mm, 3 μm）、甲酸水-甲酸乙腈为流动相和Agilent Poroshell 120 EC-C18色谱柱（4.6 mm×100 mm, 2.7 μm）、甲醇-水为流动相梯度洗脱，均采用正负离子模式采集数据。通过对照品比对及高分辨质谱解析，鉴定了其中73种化合物结构并解析了其裂解规律。7种黄酮类成分在考察的浓度范围内线性关系良好（R>0.990），回收率为96.62%~107.13%，RSD为1.06%~1.87%，在5种玫瑰花含量有显著性差异（P<0.05）。该方法快速、可靠、高效，能较全面反映并比较5种玫瑰花中非挥发性成分及7种主要黄酮类成分含量，可为深入阐明玫瑰花药效物质基础及其质量标准研究提供参考。

A sensitive and efficient method was established and validated for the qualitative and quantitative analyses of non-volatile components of five varieties of Rosa rugosa using ultra-high-performance liquid chromatography (UPLC)-QExactive Orbitrap-mass spectrometry (MS) coupled with UPLC-triple quadropole-MS/MS methods. A Thermo Hypersil Gold chromatographic column (100 mm×2.1 mm, 3 μm) with formic acid water-formic acid acetonitrile as the mobile phase and an Agilent Poroshell 120 EC-C18 chromatographic column (4.6 mm×100 mm, 2.7 μm) with methanol-water as the mobile phase were used for gradient elution. Both positive and negative ion modes were used. The structures of 73 compounds were identified and their fragmentation patterns were analyzed using high-resolution mass spectrometry. The linear relationship of the seven flavonoid components in R. rugosa was good in concentration range at which they were observed (R>0.990), and the average recovery rate was 96.62%~107.13%, with the relative standard deviation ranging from 1.06% to 1.87%. The contents of the seven flavonoids were significantly different between the five varieties of R. rugosa. This method is rapid, reliable, and efficient, and can be used to comprehensively reflect and compare the non-volatile components and seven main flavonoid contents of five varieties of R. rugosa, which can provide reference for further elucidating the material basis and quality standard of R. rugosa.

平阴县特色产业发展项目（KYC2022017）；山东省道地药材同源中药材及组方深度开发和临床转化示范研究（2012CXGC010510）