Abstract:In this study, seabuckthorn seed meal was used to prepare soluble dietary fiber using a multiple enzyme method. The structural and functional properties of the resulting fiber were comprehensively analyzed. Soluble dietary fiber was successfully obtained through a series of enzymatic hydrolysis processes, and its structural characteristics were examined in detail using techniques such as infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Additionally, the physical and chemical properties of the dietary fiber, including its water-holding capacity, oil-holding capacity, and adsorption capacity for cholesterol and glucose, were measured. The experimental results indicated that the yield of soluble dietary fiber from seabuckthorn seed meal was 5.23%. Infrared spectral analysis revealed a cellulose-like structure, while scanning electron microscopy showed a smooth and flat flaky surface, with branched structures and uniformly rounded spherical particles observed in certain areas. Regarding functional properties, the dietary fiber exhibited water-holding capacity, oil-holding capacity, and water-binding capacity of 1.1 g/g, 1.33 g/g, and 1.86 g/g, respectively. Its cholesterol adsorption capacity was 6.25 mg/g, and its glucose adsorption capacity reached 45.48 mg/g, demonstrating excellent adsorption performance. The comprehensive analysis results suggest that the soluble dietary fiber derived from sea buckthorn seed meal is structurally distinct from insoluble dietary fiber and exhibits significant activity in adsorbing cholesterol and glucose. These properties position it as a functional food ingredient with potential health benefits.
