为了对比分析不同产地（吉林、河北、云南、西藏）桑黄粗多糖的功效成分含量以及结构的差异，采用水提醇沉法对桑黄提取得到粗多糖，利用高效液相色谱、傅里叶红外光谱、紫外光谱等仪器手段对样品进行初步结构表征以及功效成分含量的对比。结果显示，西藏桑黄粗多糖的功效成分含量最高，其多糖、总酚、总黄酮、总三萜的含量分别为58.81%、11.11%、20.96%、4.49%；其热稳定性最好，热重分析后的残留量最高，质量分数达51.30%。桑黄粗多糖的单糖组成主要为葡萄糖，甘露糖，半乳糖，其多糖光谱中存在O-H, C-H等多糖特征峰，即四种粗多糖中都存在酸性多糖，且四种粗多糖的晶体状态无显著差异。该研究对比分析了四个产地的桑黄粗多糖，发现西藏桑黄粗多糖的四种功效成分含量及热稳定性最高，不同产地的桑黄粗多糖的化学成分以及结构有一定的差异，该研究为不同产地的桑黄粗多糖的开发利用提供参考。

To compare and analyze the difference of functional component content and structure of crude Phellinus igniarius polysaccharide from different origins (Jilin, Hebei, Yunnan and Xizang), the crude polysaccharides of Phellinus igniarius were extracted by hot water extraction and ethanol precipitation. The composition of monosaccharides, the preliminary characterization of structural and the content of active components were analyzed by high performance liquid chromatography, Fourier infrared spectrometer and ultraviolet-visible spectrophotometer. The results showed that the crude polysaccharide from Xizang Phellinus igniarius were had the highest content of functional components , the contents of polysaccharides, total phenols, total flavonoids and total triterpenes were 58.81%, 11.11%, 20.96%, 4.49%, respectively. It had the best thermal stability, and the residual amount after thermogravimetric analysis was the highest, and the mass fraction was 51.30%. The crude polysaccharides of Phellinus igniarius were mainly composed of glucose, mannose and galactose. There were O-H, C-H and other characteristic peaks of polysaccharides in the spectra of polysaccharides, and there were acid polysaccharides, and there was no significant difference between in crystal state. This study compared and analyzed the crude polysaccharides of Phellinus igniarius from four sources, and found that the crude polysaccharides of Xizang Phellinus igniarius had the highest content of four effective components and thermal stability. The chemical composition and structure of crude polysaccharides from different places were certain different. This study provided reference for the development and utilization of crude polysaccharides from different origins.

湖北省区域科技创新计划项目(2023EGA122)、湖北省重点研发计划项目(2021BGD016)