以植物乳植杆菌J26为研究对象，通过Biolog GEN Ⅲ Microstation自动微生物鉴定系统筛选碳氮源，优化后用25 g/L的麦芽糖及葡萄糖（1:1）混合代替MRS培养基中碳源，10 g/L的牛肉膏与酵母粉（1:1）混合代替MRS培养基中氮源。利用单因素实验确定菌株的最优发酵条件为：培养基初始pH值为5.6，发酵温度为33 ℃，接种量为4%。在此条件下，采用5 L发酵罐进行高密度发酵，植物乳植杆菌J26在发酵14 h到达稳定期，活菌数可达到4.35×1010 CFU/mL。由于，植物乳植杆菌J26活菌数高并且发酵活力强，因此可作为发酵剂，用于豆乳的发酵，可以获得具有良好活菌数（3.56×108 CFU /mL）、持水性（48.12%）和质构特性的发酵豆乳。通过优化高密度发酵工艺可以有效提高发酵液活菌数，为植物乳植杆菌J26高密度发酵工艺的应用及发酵豆乳产业化提供理论基础。

Lactiplantibacillus plantarum J26 was used as the research object. The carbon and nitrogen sources were screened using the Biolog GEN III Microstation automated microbial identification system. After optimization, a mixture of 25 g/L maltose and glucose (1:1) was used to replace the carbon source in the MRS medium, and a mixture of 10 g/L beef paste and yeast powder (1:1) was used to replace the nitrogen source in the MRS medium. The optimal fermentation conditions for the strains determined by single factor experiment were as follows: initial pH of the medium, 5.6; fermentation temperature, 33 ℃; inoculation amount, 4%. Under these conditions, a 5-liter fermenter was used for high-density fermentation. Lactiplantibacillus plantarum J26 reached the stable stage after 14 h of fermentation, and the viable cell count could reach 4.35×1010 CFU/mL. Because Lactiplantibacillus plantarum J26 has a high viable cell count and high fermentation activity, thus can be used as a starter for the fermentation of soy milk. Fermented soy milk with a good viable bacterial count (3.56×108 CFU/mL), water-holding capacity (48.12%), and textural characteristics was obtained. By optimizing the highdensity fermentation process, the number of viable bacteria in the fermentation broth can be effectively increased. This provides a theoretical basis for the application of high-density fermentation with Lactiplantibacillus plantarum J26 and the industrialization of fermented soy milk.

黑龙江省“双一流”学科协同创新成果建设项目（LJGXCG2022-017）