熟地黄多糖含量近红外模型的建立及其对肥胖小鼠肾损伤炎症的抑制作用

Near-infrared Model for Quantifying Rehmannia glutinosa Polysaccharide Content and its Anti-inflammatory Effect on Kidney Injury in Obese Mice

摘要:

建立近红外光谱法快速测定熟地黄多糖（RGP）含量的方法，并探讨不同含量RGP对肥胖小鼠炎症的影响。采集熟地黄的近红外漫反射光谱，结合偏最小二乘法（PLS），采用多元散射校正法、直接差分法和一阶导数法对光谱进行处理，以4 040.12~11 221.66 cm-1为波长区间，19为主成分数建立RGP含量的定量分析模型，其含量的预测值为34.63%~45.28%，测定值为34.49%~45.09%，绝对偏差为-0.41%~0.26%，相对偏差为0.121%~0.995%。并建立高脂饮食诱导的肥胖小鼠模型，以不同含量RGP给药14周后测定空腹血糖（FPG）、总胆固醇（TC）、甘油三酯（TG）、肌酐（Cr）和肾脏指数，观察小鼠肾脏组织病理形态。与模型组相比，RGP高含量组小鼠的体质量、TC、TG、Cr、FPG和肾脏指数分别下降11.29%、31.59%、47.63%、34.63%、28.57%、19.00%；RGP的干预可明显改善肾脏组织结构损伤，抑制炎症细胞的浸润，且作用效果与含量高低呈正相关。由此表明建立近红外光谱模型可快速准确地测定RGP含量；RGP具有调节肥胖小鼠血脂异常，抑制肾脏炎症反应，保护肾脏结构的作用，为RGP的开发利用提供实验依据。

Abstract:

A method for the rapid determination of the Rehmannia glutinosa polysaccharide (RGP) content by near infrared spectroscopy (NIRS) was established. The effects of different RGP concentrations on inflammation in obese mice were investigated. The NIR diffuse reflectance spectra of Rehmanniae Radix Praeparata were collected and analyzed using the partial least squares (PLS) method. The spectra were processed using the multivariate scatter correction (MSC), direct difference, and first order derivative methods within a wavelength range of 4 040.12~11 221.66 cm-1. The quantitative analysis model for evaluating the RGP content was established by taking into consideration 19 principal components; the predicted contents were 34.63%~45.28%, the measured contents were 34.49%~45.09%, the absolute deviations were -0.41%~0.26%, and the relative deviations were 0.121%~0.995%. The obese mice model was generated using a high-fat diet. Renal function index (RFI), renal histopathology, and fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), and creatinine (Cr) levels were evaluated after 14 weeks of RGP administration at various doses. The body weight, TC, TG, Cr, FPG, and RFI in the high-RGP content group decreased by 11.29%, 31.59%, 47.63%, 34.63%, 28.57%, and 19.00%, respectively (compared with the model group). RGP could significantly attenuate structural damage and inhibit inflammatory cell infiltration in kidney tissues, and these effects were positively correlated with the RGP content. These results showed that the NIRS model can quickly and accurately determine the RGP content, and that RGP can ameliorate dyslipidemia, inhibit the kidney inflammatory response, and protect the renal structure in obese mice. The findings of this study can help sunstantiate the development and utilization of RGP.