动物源性食品中5类兽药残留的生物芯片试剂盒检测准确性评估

Evaluation of Accuracy of Biochip Kit for Detecting Five Types of Veterinary Drug Residues in Animal-derived Foods

摘要:

该研究基于微阵列生物芯片试剂盒法评估了动物源性食品中多种兽药残留检测的结果准确性。使用微阵列生物芯片试剂盒法分别对鸡肉、鱼肉、猪肉、乳制品、蜂蜜5种动物源性食品基质中的喹诺酮类、四环素类、氟苯尼考、磺胺类和甲氧苄啶残留量进行测定，考察生物芯片试剂盒法的检出限和定量限，对结果进行准确性评估。结果显示，该方法对5种食品基质中5类兽药残留的检出限和定量限均满足要求，选用鱼肉、蜂蜜和乳制品3种基质加标进行检测，5类兽药残留的加标回收率结果均在72.58%~119.87%之间，RSD均在0.21%~22.49%内，与确证方法进行比较，未出现假阳性或假阴性结果，准确性较好。结果表明，生物芯片试剂盒法检测速度快、成本低、结果准确可靠，在动物源性食品多兽药残留同时筛检中具有广阔应用前景。

Abstract:

In the study, the accuracy of the detection results of multiple veterinary drug residues in animal-derived foods was evaluated based on the microarray biochip kit method. The microarray biochip kit method was used to determine the residues of quinolones, tetracyclines, florfenicol, sulfonamides, and trimethoprim in five animal-derived food matrices, including chicken, fish, pork, dairy products, and honey. The detection and quantification limits of the biochip kit method were examined, and the accuracy of the results was evaluated. The results showed that the detection limit and quantification limit of the 5 types of veterinary drug residues in the 5 food matrices all met the requirements. Three matrices including fish, honey, and dairy products were selected for spiking detection, and the spiked recovery rates of the 5 types of veterinary drug residues were in the range of 72.58%~119.87%, with RSD values ranging from 0.21% to 22.49%. Compared with the confirmation method, there were no false positive or false negative results, and the accuracy was good. The results indicate that the biochip kit method is rapid, requires low cost, and has accurate and reliable results and broad application prospects in simultaneous screening of multiple veterinary drug residues in animal-derived foods.