脉冲电场（PEF）作为一种新型的物理场非热加工技术，其在流体食品的杀菌、灭酶等方面已开展大量的应用研究。近年来，基于PEF“电穿孔”对细胞优越的破壁效果，其在细胞的穿孔、强化胞内功能性成分的提取已逐渐成为研究热点。该文以生姜为对象，首先对比了不同提取工艺［传统溶剂浸提法（TET）、超声辅助提取法（UET）、索氏提取法（SET）、脉冲电场辅助提取法（PEF）］对生姜姜酚提取量的影响，然后利用响应面法优化了PEF强化生姜姜酚的提取参数。结果表明，与TET、UET、SET相比，PEF处理对生姜姜酚提取量最高，达13.36 mg/g•DW。进一步通过单因素和响应面试验设计，获得的最佳工艺参数为电场强度：2.0 kV/cm，脉冲次数：22个，脉冲宽度：19 μs。此优化条件下，姜酚提取量为14.69 mg/g•DW，比传统溶剂浸提法的9.81 mg/g•DW，提高了49.75%。该研究表明PEF在强化生姜姜酚提取优于其它提取工艺，且具有良好的应用前景。

Pulsed electric field (PEF) is a novel non-thermal physical field processing technology used in the sterilization and enzyme inactivation of fluid foods, presenting an substantial amount of research on its applications. In recent years, the superior cell wall-breaking capability of PEF, known as “electroporation,” has attracted increasing attention, and its ability to enhance wall breaking and intracellular functional component extraction has gradually become a popular research topic. The extraction rates of gingerol from ginger were compared by different extraction processes (traditional solvent extraction technique [TET], ultrasonic-assisted extraction technique [UET], soxhlet extraction technique [SET], and pulsed electric field assisted extraction [PEF]). The parameters for the PEF method of extracting gingerol was then optimized by the response surface method. Results indicated that compared to TET, UET, and SET, the PEF method produced the highest extraction yield of gingerol, reaching 13.36 mg/g•DW. Further steps were taken based on single factor and response surface experiment design, and the optimal process parameters for extracting gingerol from ginger by PEF were obtained as follows: electric field intensity: 2.0 kV/cm, pulse frequency: 22, and pulse width: 19 μs, Under this optimal condition, the extracted amount of gingerol was mg/g•DW, an increase of 49.75% compared to the traditional solvent extraction method (9.81 mg/g•DW). This study shows that PEF is the superior extraction methods in enhancing gingerol extraction from ginger and with great application prospects.

湖南省高新技术产业科技创新引领计划项目（2022Gk4014）；湖湘青年英才（2020RC3097）