Abstract:Polygonatum sibiricum and raspberry are traditional ingredients both for medicinal purposes and in food, featuring good antioxidant effects. A nutritious health beverage was developed using P. sibiricum and raspberry extracts as the main ingredients, with Polygonatum polysaccharide and total raspberry flavonoids as the main active ingredients. An evaluation of the in vitro antioxidant effect of this health beverage was performed. Orthogonal tests showed that the highest Polygonatum polysaccharide extraction yield could reach 17.94% when using ultrasonic treatment at a temperature of 60 ℃, 80 min, and a solid-liquid ratio of 1:20. The total raspberry flavonoids were also extracted, with the highest extraction yield at 5.36% when 0.9 g/L pectinase was added, the enzymolysis time and temperature were 225 min and 45 ℃. The optimal formula of the health beverage was determined using a response surface experiment, designed as follows: The ratio of P. sibiricum and raspberry extract was 3:7, concentration of xylitol was 60 g/L, and concentration of citric acid was 0.3 g/L. The sensory evaluation score for this formula was 94 points. The results of the antioxidant test in vitro showed that the scavenging rate of Polygonatum-raspberry health drink to DPPH free radicals was 79.35%, scavenging capacity to ABTS+ free radicals was 79.35 mmol/L Trolox equivalent, and antioxidant capacity measured by the FRAP method was 1.56 mmol/L FeSO4 standard equivalent. In addition, the results of the cell counting kit-8 (CKK-8) assay also indicated that the Polygonatum sibiricum-raspberry health beverage significantly mitigated the H2O2-induced damaging effects of PC12 cells, restoring the cell proliferation rate from 61.55% to 81.37%. This health drink exhibited good antioxidant effects, provides a foundation for follow-up research and development of other P. sibiricum and raspberry health products, also promoting local P. sibiricum and raspberry industries.
