黄精、覆盆子是传统的药食两用资源，具有很好的抗氧化功效。该研究以黄精多糖和覆盆子总黄酮为主要活性成分指标，以黄精提取液和覆盆子提取液为主要成分，开发一种养生保健饮料，并对该款保健饮料进行体外抗氧化功效评价。正交试验结果显示，在超声温度60 ℃、超声时间80 min、料液比1:20时黄精多糖得率最高可达17.94%；在果胶酶添加量为0.90 g/L、酶解时间225 min、酶解温度45 ℃时覆盆子总黄酮的得率最高可达5.36%。通过响应面实验设计确定饮料的最佳配方：黄精和覆盆子提取液配比为3:7、木糖醇60 g/L、柠檬酸0.3 g/L，此条件下感官评价为94分。体外抗氧化实验结果显示，黄精覆盆子保健饮料对DPPH自由基的清除率可达79.35%，对ABTS+自由基的清除能力为79.35 mmol/L Trolox当量，FRAP法测定的抗氧化能力为1.56 mmol/L FeSO4 当量。此外，CKK-8法结果也表明黄精覆盆子保健饮料能显著缓解H2O2诱导的PC12细胞损伤作用，使细胞增值率从61.55%恢复至81.37%。该款保健饮料呈现出良好的抗氧化功效，为黄精覆盆子保健新产品的研发提供了新思路，同时也促进了黄精覆盆子产业在本地区的推广。

Polygonatum sibiricum and raspberry are traditional ingredients both for medicinal purposes and in food, featuring good antioxidant effects. A nutritious health beverage was developed using P. sibiricum and raspberry extracts as the main ingredients, with Polygonatum polysaccharide and total raspberry flavonoids as the main active ingredients. An evaluation of the in vitro antioxidant effect of this health beverage was performed. Orthogonal tests showed that the highest Polygonatum polysaccharide extraction yield could reach 17.94% when using ultrasonic treatment at a temperature of 60 ℃, 80 min, and a solid-liquid ratio of 1:20. The total raspberry flavonoids were also extracted, with the highest extraction yield at 5.36% when 0.9 g/L pectinase was added, the enzymolysis time and temperature were 225 min and 45 ℃. The optimal formula of the health beverage was determined using a response surface experiment, designed as follows: The ratio of P. sibiricum and raspberry extract was 3:7, concentration of xylitol was 60 g/L, and concentration of citric acid was 0.3 g/L. The sensory evaluation score for this formula was 94 points. The results of the antioxidant test in vitro showed that the scavenging rate of Polygonatum-raspberry health drink to DPPH free radicals was 79.35%, scavenging capacity to ABTS+ free radicals was 79.35 mmol/L Trolox equivalent, and antioxidant capacity measured by the FRAP method was 1.56 mmol/L FeSO4 standard equivalent. In addition, the results of the cell counting kit-8 (CKK-8) assay also indicated that the Polygonatum sibiricum-raspberry health beverage significantly mitigated the H2O2-induced damaging effects of PC12 cells, restoring the cell proliferation rate from 61.55% to 81.37%. This health drink exhibited good antioxidant effects, provides a foundation for follow-up research and development of other P. sibiricum and raspberry health products, also promoting local P. sibiricum and raspberry industries.

丽水市本级重点研发计划项目（2021ZDYF07）；浙江省自然科学基金探索青年项目（LQ23C200002）；丽水市公益性技术应用研究计划项目（2023GYX45）；丽水市科技特派员项目（2020TPY20）