Abstract:Milk beer is a new type of fermented milk beverage made by two-step fermentation of lactic acid bacteria and yeast. The feasibility of producing milk beer from reconstituted milk was analyzed in terms of physicochemical properties, water holding capacity(WHC) ,color, particle size, texture, rheology, scanning electron microscopy, and aroma composition, etc. The quality of milk beer prepared from reconstituted milk was slightly reduced compared with that of fresh milk beer, but it was not significant (P>0.05). The water holding capacity of reconstituted milk beer decreased by 1.17%; the brightness L* value decreased by 24.8%; the protein particle size increased by 15.3%; and there was a small difference in aroma with that of raw milk beer; rheological analysis showed that with the extension of shear time, the apparent viscosity of reconstituted milk beer and raw milk beer had the same trend of change; scanning electron microscopy showed that there was no significant difference in the microstructure of the reconstituted milk beer; and the sensory evaluation score of the reconstituted milk beer was 7.50, which was slightly lower than that of raw milk beer; in terms of texture, the elasticity of reconstituted milk beer increased by 8.92% compared with that of raw milk beer, and there were no significant differences in hardness and cohesion (P>0.05), which indicated that these differences could be accepted by consumers. This study proves that reconstituted milk can replace fresh milk fermented milk beer to a certain extent, and solves the problem of over-reliance on fresh milk in milk beer making, but the fresh milk in the mainland is insufficient and the quality varies.