Abstract:Fat-soluble vitamins are essential micronutrients for human growth and development, and fat-soluble vitamin deficiencies pose a serious threat to human health. Furthermore, the most of fat-soluble vitamins cannot be synthesized in the human body and must be obtained from external sources. Therefore, the rapid and sensitive detection of fat-soluble vitamins is crucial to ensure the safety of vitamin intake. Although traditional methods (such as high-performance liquid chromatography (HPLC), high performance liquid chromatography and tandem mass spectrometry (LC-MS), and supercritical fluid chromatography (SFCS)) has the advantages of high selectivity and sensitivity. Nevertheless, the disadvantages of complex sample pre-treatment, time-consuming, and reliance on large-scale instrumentation have limited the applicability of traditional methods in the rapid detection of fat-soluble vitamins. Recently, biosensors have been applied to the detection of fat-soluble vitamins due to their high specificity, high sensitivity, low cost, on-site analysis, and ease of handling. This review first summarized the physiological functions of common fat-soluble vitamins. Secondly, the advantages and disadvantages of traditional methods and biosensors for detection of fat-soluble vitamins were discussed. Finally, the latest research progress of biosensors in the detection of fat-soluble vitamins was comprehensively summarized and discussed. This paper mainly summarizes the biological components, linear sensing range, sensitivity and detection limit of lipid-soluble vitamin detection biosensors, providing reference and basis for the development of future lipid-soluble vitamin detection biosensors.
