探究了麦角甾醇亚油酸酯（简称麦角甾醇酯）的酶促合成及对非酒精性脂肪肝的预防作用。将30只雄性昆明小鼠分为四组：空白组（n=6）、高脂组（n=8）、麦角甾醇组（n=8）和麦角甾醇亚油酸酯组（n=8），持续喂养6周。测定血浆总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平，计算LDL-C/HDL-C比值，检测肝脏和粪便脂质水平，并分析肝脏脂肪变性程度。结果显示，以Candida sp. 99-125脂肪酶为催化剂可以成功合成麦角甾醇亚油酸酯。麦角甾醇酯使得肝脏总脂肪酸含量降低35.3%（P<0.05），可以有效预防高脂膳食诱导肝脏脂肪变性，并导致粪便总脂肪酸水平增加74.8%（P<0.05）。同时，麦角甾醇酯降低了血浆LDL-C水平和LDL-C/HDL-C比值分别为24.3%（P<0.05）和36.5%（P<0.05），引起血浆HDL-C水平提高52.0%（P<0.05）。以上结果表明，麦角甾醇酯能够有效预防高脂膳食诱导的非酒精性脂肪肝。该文为非酒精性脂肪肝的预防提供了一种新的干预策略。

The lipase-catalyzed synthesis of ergosterol linoleate was explored, along with its potential role in mitigating non-alcoholic fatty liver disease (NAFLD). Thirty male Kunming mice were divided into four groups: blank (n=6), high-fat (n=8), ergosterol (n=8), and ergosterol linoleate (n=8). The mice were fed for six weeks. Plasma levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were determined. LDL-C/HDL-C ratio was calculated, and lipid levels in the liver and feces were measured, along with the degree of liver fat degeneration. The results showed that ergosterol linoleate could be successfully synthesized using Candida sp. 99-125 lipase as a catalyst. Ergosterol ester reduced the total fatty acid content in the liver by 35.3% (P<0.05), effectively preventing high-fat diet-induced liver fat degeneration, and increased fecal total fatty acid levels by 74.8% (P<0.05). Meanwhile, ergosterol ester decreased plasma LDL-C levels and LDL-C/HDL-C ratio by 24.3% (P<0.05) and 36.5% (P<0.05), respectively, and increased plasma HDL-C levels by 52% (P<0.05). These results indicate that ergosterol ester can effectively prevent high-fat diet-induced NAFLD. This study provides a new dietary intervention strategy for the prevention of NAFLD.

新疆自治区区域协同创新专项-科技援疆计划（2022E02094）；攀西特色作物研究与利用四川省重点实验室开放课题（SZKF2211）；食品营养与安全湖北省重点实验室开放基金（FN S-H BKL2023A01）；江苏大学2023年校级大学生创新训练计划项目（202310299647X）