为了实现红枣多糖的高效制备及开发利用，该研究建立了红枣多糖超高压耦合低共熔溶剂提取工艺，并探究了红枣多糖的抗氧化活性。以红枣为原料，从七种不同类型的低共熔溶剂（Deep Eutectic Solvent, DES）体系中，筛选确定以氯化胆碱和乙二醇组成的DES提取红枣多糖，在单因素试验的基础上，采用Box-Benhnken中心组合试验设计优化红枣多糖的超高压耦合DES提取工艺，建立四因素回归模型，确定其最优提取工艺条件为提取压力483 MPa，保温时间8.25 min，DES中氯化胆碱和乙二醇的摩尔比为1:3.75，DES含水量为53%，此时红枣多糖实际得率为14.22%。同时该工艺制备得到的红枣多糖具有较高的DPPH、ABTS+自由基清除作用，以及铁离子和氧自由基还原能力，分别为1.47 mg AA/g DW、10.69 mg AA/g DW、30.43 μmol FeE/g DW和193.67 μmol TE/g DW，具有显著的抗氧化活性，表明超高压耦合DES提取工艺具有制备高得率、高抗氧化活性红枣多糖的潜力。

To realize the efficient preparation and utilization of jujube polysaccharides, a deep eutectic solvent (DES)-based ultrahigh pressure extraction technique was established, and the antioxidative activity of the polysaccharides was investigated. Seven different types of DES systems were tested for their effectiveness in extracting polysaccharides from pretreated jujube powder. Based on single-factor tests, the Box-Behnken central composite design was used to optimize the extraction process. According to the results, the optimal DES system for extracting jujube polysaccharides consisted of choline chloride and ethylene glycol. The four-factor regression model established in this study determined the optimal extraction conditions to be as follows: extraction pressure, 483 MPa; duration, 8.25 min at a constant temperature; molar ratio of choline chloride to ethylene glycol, 1:3.75; and water content of the DES system, 53%. Under these conditions, the actual extraction yield of jujube polysaccharides was 14.22%. The obtained polysaccharides exhibited high antioxidative activity, with a DPPH radical scavenging activity of 1.47 mg AA/g DW, an ABTS radical scavenging activity of 10.69 mg AA/g DW, a FRAP value of 30.43 μmol FeE/g DW, and an ORAC value of 193.67 μmol TE/g DW. The results showed that the DES based ultrahigh pressure extraction process has the potential to realize the high-yielding production of jujube polysaccharides with excellent antioxidative activity.

广东省重点研发计划项目（2022B0202020004）；广东省特支计划本土创新团队项目（2019BT02N112）