解析具有调节血糖功能的食品组合物桑瓜饮（Sang-Gua-Yin, SGY）的成分。使用高效液相色谱（High Performance Liquid Chromatography, HPLC）归属桑叶总生物碱组分、苦瓜总皂苷组分、葛根总黄酮组分的色谱峰，高效液相色谱-蒸发光散射器（High Performance Liquid Chromatography-Evaporative light Scattering Detector, HPLCELSD）与液相色谱-串联质谱法（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）分析组合物桑瓜饮中的化学成分。对食品组合物桑瓜饮的色谱峰进行归属，标定了11个色谱峰，其中7种成分属于葛根总黄酮，3种成分属于苦瓜总皂苷，1种成分属于桑叶总生物碱；对16个化学成分进行了鉴定，分别为1-DNJ、苦瓜苷A、苦瓜苷K、葛根素、6′′-O-acetyldaidzin、pueroside D、染料木苷、大豆苷、大豆苷元、3′-羟基葛根素、3′-甲氧基葛根素、3′-氢化葛根素木糖苷、葛根素-7-木糖苷、葛根素-6′′-O-木糖苷、puerarin-7-O-glucoside、葛根素-4′-O-β-D-葡萄糖苷。该研究提示食品组合物桑瓜饮成分选择1-DNJ、苦瓜苷A与葛根素作为评价指标，为食品组合物桑瓜饮的后续开发利用提供科学依据。

Sang-Gua-Yin (SGY) is a food preparation with the ability to regulate blood glucose. High Performance Liquid Chromatography (HPLC) was used to attribute the chromatographic peaks of the total alkaloids from Folium Mori, total saponins from Fructus Momordicae Charantiae and total flavonoids from Radix Puerariae Lobatae. The chemical components in the composition of SGY were analyzed by High Performance Liquid Chromatography-Evaporative light Scattering Detector (HPLC-ELSD) and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). The chromatographic peaks of the composition of SGY were attributed to 11 peaks, among which seven components belonged to total flavonoids of Radix Puerariae lobatae, three components to total saponins of Fructus Momordicae charantiae, and one component to total alkaloids of Folium mori. In addition, 16 chemical components were identified as 1-DNJ, momordicoside A, momordicoside K, puerarin, 6′′-O-acetyldaidzin, pueroside D, genistein, daidzin, daidzein, 3′-hydroxypuerarin, 3′-methoxy puerarin, 3′-hydrogenated glucosin xyloside, glucosin-7-xyloside, glucosin-6′′-O-xyloside, puerarin-7-O-glucoside, and glucosin-4′-O-β-D-glucoside. These results indicate that 1-DNJ, momordicoside A, and puerarin could be used as indices with which to evaluate for the ingredients of SGY, providing a scientific basis for the subsequent development and utilization of SGY.

湖北省自然科学基金创新发展联合基金（2024AFD237）；教育部产学合作协同育人项目（230804643304437）；湖北省科学技术厅揭榜制科技项目（2023BEB011）