不同品种金槐米代谢组学比较

Comparison of differential metabolomics among different types of Sophora japonica ‘Jinhuai’

摘要:

为探明不同成熟期金槐代谢组学的差异,本研究利用液相色谱与质谱联用仪(LC-MS)对4种不同类型金槐的代谢物进行了测定。结果表明,金槐槐米中测定的代谢物共有1135个化合物,632个为差异代谢物；4个类型共有62个重复差异代谢物,主要为脂质和类脂分子(占30%)、有机杂环化合物(占23.33%)、有机酸及其衍生物(占20%)、有机含氧化合物、苯丙素类化合物(各占10%)、生物碱和衍生物(占6.67%)等6类大物质；差异代谢物主要是苯丙氨酸、酪氨酸和色氨酸生物合成通路富集显著；4类金槐槐米均富含芦丁、槲皮苷,基于黄酮类化合物组成聚类,金槐J6和金槐J3各为单独一类,金槐J2和金槐J4为一类；金槐米含有丰富的代谢物,早熟和中熟槐米代谢物组成相似度高,晚熟槐米代谢物组成与其他两类差异明显,差异代谢物主要集中在芳香族氨基酸的生物合成通路。

Abstract:

In order to investigate the differences in metabolomics at different mature stages, the metabolites of 4 different types of Sophora japonica were determined by liquid chromatography-mass spectrometry (LC-MS). The results showed that there were 1135 metabolites in Jinhuai rice, 632 of which were differential metabolites. There were 62 repeated differential metabolites among 4 types, which were mainly lipids and lipid-like molecules (30%), organic heterocyclic compounds (23.33%), organic acids and their derivatives (20%), organic oxygen compounds, phenylpropanoids (10% each), alkaloids and derivatives (6.67%), etc. The different metabolites were mainly enriched in the biosynthetic pathways of phenylalanine, tyrosine, and tryptophan. The four kinds of Sophora japonica rice were rich in rutin and quercetin, and based on flavonoid composition clustering, Sophora Japonica J6 and Sophora Japonica J3 were separate ones, and Sophora Japonica J2 and Sophora Japonica J4 belonged to the same group. Golden Acacia rice contained abundant metabolites, and the metabolite composition of early- and mid-maturing Acacia rice had high similarity, while the metabolite composition of late-maturing Acacia rice was significantly different from the other two types, and the difference of metabolites mainly focused on the biosynthesis pathway of aromatic amino acids.