该文探讨了人参皂苷Rg3对氧化应激诱导PC12细胞损伤的保护作用。通过细胞抗氧化活性（CAA）实验检测Rg3对氧自由基诱导细胞氧化的抑制作用，并建立H2O2诱导的PC12细胞氧化应激模型，CCK-8法检测Rg3对细胞的保护和毒性作用，运用Western blot检测Rg3对炎症和凋亡相关信号通路中p65磷酸化蛋白和CREB磷酸化蛋白的影响。结果发现Rg3对氧自由基的抗氧化能力约是Trolox的7.3倍。200 μmol/L H2O2诱导PC12氧化应激时细胞活力下降至60.11%，p-p65蛋白相对量为1.30，p-CREB蛋白相对量为0.80。10、20和40 μmol/L Rg3保护组的细胞活力分别提高至74.00%、76.04%、80.36%，呈浓度依赖式提高；细胞p-p65蛋白相对量分别降低至1.18、1.03、0.82；细胞p-CREB蛋白相对量分别提高至1.31、1.75、2.30。结果表明人参皂苷Rg3可能通过清除氧自由基，参与NF-κB/CREB通路调控，减轻PC12细胞炎症和凋亡，抑制氧化应激诱导的神经细胞损伤，为人参的神经营养特性研究及相关功能食品开发提供理论依据。

To determine protective effect of ginsenoside Rg3 on oxidative stress-induced injury in PC12 cells, the inhibitory effect of Rg3 on oxygen free radicals induced cell oxidation was detected in a cellular antioxidant activity experiment. To this end, an oxidative stress model of PC12 cells induced by H2O2 was established, whose cell viability was detected using the CCK-8 method. The effects of Rg3 on the protein expression of p65 and CREB, related to inflammatory and apoptosis, were detected by western blotting. The results showed that the antioxidant ability of Rg3 in response to oxygen free radicals was approximately 7.3-fold that of Trolox. Cell viability was decreased by 60.11% after treatment with 200 μmol/L H2O2. Meanwhile, the relative expression of p-p65 protein was increased to 1.30, and p-CREB protein was decreased to 0.80. The cell viabilities of protective groups pretreated with different concentrations of Rg3 (10, 20, and 40 μmol/L) were found to increase by 74.00%, 76.04%, and 80.36%, respectively. The relative expressions of p-p65 protein decreased to 1.18, 1.03, and 0.82, respectively. Meanwhile, the relative expression of p-CREB protein increased to 1.31, 1.75 and 2.30, respectively. These results suggest that ginsenoside Rg3 may protect PC12 cells from oxidative damage by scavenging oxygen free radicals. The underlying mechanisms may be related to the NF-κB/CREB pathway, which reduces inflammation and apoptosis in PC12 cells, thereby inhibiting the oxidative stress-induced nerve cell damage. These findings provide a theoretical basis for the study of the neurotrophic characteristics of ginseng and the development of related functional foods.

吉林省自然科学基金项目（20200201146JC）