基于UHPLC-Q-Exactive-MS分析不同香型信阳毛尖茶中的非挥发性成分
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1.信阳农林学院;2.中国农业科学院茶叶研究所;3.信阳市文新茶叶有限责任公司;4.信阳农林学院茶学院;5.光山县大苏山茶产业服务中心

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Analysis of non-volatile compounds among Xinyang Maojian tea with different aroma types based on UHPLC-Q-Exactive-MS
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    摘要:

    采用超高效液相色谱-四极杆轨道阱质谱(Ultra-high performance liquid chromatography-Q-Exactive-mass spectrometry, UHPLC-Q-Exactive-MS)技术分析清香、兰花香和嫩玉米香型信阳毛尖茶中非挥发性化学成分的差异。从不同香型信阳毛尖茶中共鉴定得到177个化合物,其中有24个差异化合物,包括11个黄酮类、7个氨基酸及其衍生物、2个糖类及其衍生物和4个其他类化合物。清香型和嫩玉米香型之间有7个差异化合物,其中5个为黄酮类化合物;清香型和兰花香型之间有9个差异化合物,其中6个是L型氨基酸及其衍生物。KEGG代谢通路分析表明,不同香型信阳毛尖茶之间的非挥发性差异代谢物的合成和代谢主要与丙氨酸、天冬氨酸和谷氨酸代谢途径和黄酮类化合物的生物合成途径密切相关。L-缬氨酸、L-异亮氨酸和L-亮氨酸在不同香型信阳毛尖茶之间的差异对其香气的形成有重要贡献。了解不同香型信阳毛尖茶中非挥性化合物的差异,对提升信阳毛尖茶的精准加工技术具有重要指导意义。

    Abstract:

    The differential non-volatile compounds among Xinyang Maojian tea (XYMJ) with refreshing, orchid-like and tender corn-like aroma were identified by ultra-high performance liquid chromatography-Q-Exactive-mass spectrometry (UHPLC-Q-Exactive-MS). A total of 177 chemical compounds were identified in the XYMJ teas with different aroma types, 24 of which were found to show significantly different among the XYMJ tea samples, including 11 flavonoids, 7 amino acids and their derivatives, 2 sugars and their derivatives, and 4 other kinds. There were 7 differential compounds between the refreshing and tender corn-like XYMJ teas, 5 of which were the flavonoids. There were 9 differential compounds between the refreshing and orchid-like XYMJ teas, 6 of which were the L-amino acids and their derivatives. KEGG metabolic pathway enrichment analysis showed that the differential compounds were significantly enriched in the pathways of alanine, tyrosine and tryptophan biosynthesis, and flavonoids biosynthesis. L-valine, L-isoleucine and L-leucine were found to show significantly different among the XYMJ teas with different aroma types, which contributed importantly to the XYMJ tea aroma formation. Understanding the differences of non-volatile compounds among the XYMJ teas with different aroma types had important implications for promoting the precious processing technology of XYMJ teas.

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  • 收稿日期:2024-07-21
  • 最后修改日期:2024-10-11
  • 录用日期:2024-10-21
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