[关键词]
[摘要]
为探究外源茉莉酸甲酯(MeJA)对鲜莲子采后品质的影响,以清水为对照,用100 μmol/L MeJA浸泡处理鲜莲子,每隔2 d比较莲子的色差(L*、a*、b*)、营养物质(淀粉、可溶性糖)和其他生理生化指标(失重率、总酚、丙二醛、过氧化氢以及多酚氧化酶、超氧化物歧化酶、过氧化氢酶、过氧化物酶)的变化。结果表明:相比对照组,MeJA处理可有效维持鲜莲子采后色泽。贮藏8 d时处理组的失重率降低了33.84%,淀粉含量提高了38.34 mg/g,可溶性糖含量提高了70.58 mg/g;同时,MeJA处理可减少多酚含量,抑制PPO活性和MDA、H2O2的积累,提高SOD、CAT和POD活性。以上结果显示MeJA可通过减少鲜莲子采后的物质消耗、维持淀粉和可溶性糖含量、减少酚类的氧化、提高抗氧化酶酶活、维持细胞膜完整性等延缓鲜莲子的采后品质衰退,有助于延长其货架期。
[Key word]
[Abstract]
To investigate the effects of exogenous methyl jasmonate (MeJA) on the post-harvest quality of fresh lotus seed, MeJA (100 μmol/L) was used to soak the fresh lotus seed with water as the control. The changes in the color difference (L*, a*, b*), nutrient content (starch, soluble sugar), and other physiological and biochemical indexes (weight loss rate, total phenol content, malondialdehyde content, hydrogen peroxide content, polyphenol oxidase activity, superoxide dismutase activity, catalase activity and peroxidase activity) were compared every 2 days. The results showed that compared with the control group, the MeJA treatment effectively maintained the postharvest color of fresh lotus seed. After 8 days of storage, compared with the control group, the MeJA-treated group had a lower weight loss rate (decreased by 33.84%), and higher starch content (increased by 38.34 mg/g), higher soluble sugar content (increased by 70.58 mg/g), along with a reduced total phenol content, suppressed PPO activity, MDA content and H2O2 accumulation, and enhanced SOD, CAT and POD activities. The above results revealed that the MeJA treatment could delay the post-harvest quality decline of fresh lotus seed through reducing material consumption, maintaining starch and soluble sugar contents, reducing the oxidation of phenols, improving the activities of antioxidant enzymes, as well as maintaining the cell membrane integrity, thereby prolonging their shelf life.
[中图分类号]
[基金项目]
湖北省教育厅中青年项目(Q20202702)