[关键词]
[摘要]
为明确彩色马铃薯新品种闽彩薯1号、闽彩薯2号、闽彩薯3号和闽彩薯4号的块茎花青素组分和含量的差异,为新品种选育及花青素开发应用提供依据。利用高效液相色谱与质谱联用技术,进行了块茎花青素组分的定性与比较分析。结果表明,4个彩色品种共含有14种花青素单体组成,其中闽彩薯1号含有其中的6种组分,闽彩薯2号含有其中的9种组分,闽彩薯3号含有其中的10种组分,3个紫肉色马铃薯花青素组分主要是矮牵牛素3-p-香豆酰基芸香糖苷-5-葡萄糖苷和芍药素3-p-香豆酰基芸香糖苷-5-葡萄糖苷。闽彩薯4号含有5种花青素组分,主要组分为矢车菊素3-芸香糖苷和天竺葵素3-p-香豆酰基芸香糖苷-5-葡糖苷,紫肉色马铃薯花青素组分与红肉色马铃薯青素组分具有明显差异。4个彩色马铃薯花青薯含量分别为139.77、95.48、78.08、67.25 mg/100 g FW,品种之间具有显著差异。同时,这4个彩色马铃薯组分含有全部6种花青素基本苷元,且花青素结构较为稳定,可作为重要的天然色素的原料及杂交育种的亲本材料。
[Key word]
[Abstract]
In order to clarify the differences in anthocyanin components and contents of new potato varieties with colored flesh, Mincaishu 1, Mincaishu 2, Mincaishu 3 and Mincaishu 4, and provide a foundation for the screening, and cultivation of novel cultivars and the development and application of anthocyanins. Qualitative and comparative analyses of the anthocyanin components in the tubers were conducted by using high performance liquid chromatography coupled with mass spectrometry. The results showed that there were fourteen anthocyanin monomers in the four cultivars, among which, Mincaishu No. 1 contained six types of monomers, Mincaishu No. 2 had nine types of monomers, and Mincaishu No.3 contained ten types of monomers. The main anthocyanin components of the three purple-fleshed potatoes were delphinidin 3-p-coumaroylrutionoside-5-glucoside and peonidin 3-p-feruloylrutinoside-5-glucoside. Mincaishu No.4 contained five types of anthocyanin components, with cyanidin 3-rutinoside and pelargonidin 3-p-coumaroylrutinoside-5-glucoside were the main components. There were significant differences in the anthocyanin components between the purple-fleshed potato and the red-fleshed potato. The anthocyanin contents in the four potato varieties with colored flesh were 139.77, 95.48, 78.08 and 67.25 mg/100 g FW, respectively, indicating the vast differences among the varieties. The four varieties contained all the six fundamental anthocyanin aglycones and their anthocyanin structures were relatively stable, which can serve as important raw materials of natural pigments and as the parent materials for hybrid breeding.
[中图分类号]
[基金项目]
福建省属公益类科研院所基本科研专项(2021R1031007);薯类作物科技创新团队(CXTD2021012-1);中国农业科学院“5511”协同创新工程(XTCXGC2021005);国家现代农业产业技术体系福州综合试验站项目(CARS-09-ES11)