护肝醒酒软糖混合液对小鼠急性酒精性肝损伤的保护作用

Protective Effect of Hepatoprotective and Sobering Soft Candy Mixture on Acute Alcoholic Liver Injury in Mice

摘要:

探究护肝醒酒软糖混合液（LSSCM）对小鼠急性酒精性肝损伤的保护作用。建立急性酒精性肝损伤模型，计算肝脏指数，测定血清中甘油三酯（TG）、丙氨酸氨基转移酶（ALT）、谷草转氨酶（AST）和肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）的水平变化及通过肝组织病理切片分析其形态变化。结果表明，与模型组相比，LSSCM高剂量组肝脏指数、TG含量、MDA含量显著降低12.33%、48.35%、39.10%；SOD、ALDH、ADH水平显著提高18.61%、44.59%、124.61%；AST、ALT 水平显著降低32.26%、40.71%；小鼠肝组织病理切片结果显示，LSSCM各剂量组小鼠受损肝脏均得到不同程度改善。综上所述，LSSCM对酒精所致的急性肝损伤小鼠的肝脏肿大、脂质代谢紊乱、肝脏氧化应激具有显著改善效果，可有效预防和改善急性酒精性肝损伤。

Abstract:

To study the protective effect of the Liver-protective and Sobering Soft Candy Mixture (LSSCM) against acute alcoholic liver injury in mice, a model of acute alcoholic liver injury was established, the liver index was calculated, and the changes in serum triglycerides (TG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as the superoxide dismutase (SOD), malondialdehyde (MDA), alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in liver tissues, were measured. morphological changes were examined through pathological sections of liver tissues. The results showed that compared with the model group, the liver index, TG content and MDA content of the high-dose LSSCM group were significantly reduced by 12.33%, 48.35% and 39.10%, respectively; the levels of SOD, ALDH and ADH increased significantly by 18.61%, 44.59% and 124.61%, respectively; the levels of AST and ALT were significantly reduced by 32.26% and 40.71%, respectively. The results of mouse liver histopathological sections showed that the damaged livers of mice in each dose group of LSSCM were improved to different degrees. In summary, LSSCM had a significant ameliorating effect on hepatomegaly, lipid metabolism disorders, and hepatic oxidative stress caused by alcohol-induced acute liver injury in mice, and could effectively prevent and ameliorate acute alcoholic liver injury.