Abstract:In recent years, a large number of studies have shown that intestinal flora is a key influencing factor of human health, and some dietary fibers such as inulin and polydextrose play an important role in regulating intestinal health. In this paper, starting from the in vitro digestive simulation experiment, we found that the maximum hydrolysis degree of resistant dextrin in simulated gastric fluid and simulated intestinal fluid was 1.3% and 3.7%, respectively, which showed obvious anti-digestive effects, and verified the physiological properties of L P resistant dextrin. The in vitro intestinal probiotic culture study of L P resistant dextrin found that Lactobacillus bulgaricus and Lactobacillus casei were unable to utilize the resistant dextrin as a single nutrient source for their growth, but the addition of a certain amount of resisted dextrin on the basis of a certain nutrient source could promote the proliferation of the two kinds of probiotic bacteria, and the optimal amount of its addition was 0.6% and 0.4% in order. In addition, by comparing different dietary fibers on the growth of probiotics in vitro, it was found that polydextrose, inulin, and oligofructose were able to promote the proliferation of probiotics faster, while resistant dextrin promoted the growth to a greater concentration, and Lactobacillus casei exhibited a higher utilization of resistant dextrin compared to Lactobacillus bulgaricus. Thus, it can be seen that L P resistant dextrin not only has slow digestive properties in vitro, but also effectively promotes the proliferation of beneficial flora in in vitro culture experiments, showing obvious prebiotic effects.