抗性糊精调节两种肠道益生菌体外增殖
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1.广东广业清怡食品科技股份有限公司;2.华南理工大学食品科学与工程学院;3.华南理工大学轻工科学与工程学院

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2022 年度广东省重点领域研发计划项目-“低热量糊精生物加工新技术与产品开发”(项目编号:2022B0202020002)


Resistant dextrin regulating the proliferation of two intestinal probiotics in vitro
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    摘要:

    肠道菌群是人体健康的关键影响因子,膳食纤维在调节肠道菌群平衡方面有着重要作用。该文从清怡牌抗性糊精体外消化实验出发,探究抗性糊精对保加利亚乳杆菌和干酪乳杆菌体外生长的影响,对比研究了抗性糊精与其它膳食纤维对两种益生菌体外增殖的差异。结果表明,在模拟胃液和肠液中清怡牌抗性糊精最大水解度分别为1.3%和 3.7%,表明抗性糊精具有良好的耐胃肠道消化特性。抗性糊精虽然无法作为单一营养碳源生长促进两种益生菌增殖,但在保证营养源条件下,添加一定量抗性糊精能够促进菌种体外生长,在基础培养基中较优的抗性糊精添加量分别为0.6%和0.4%(w/w)。而且,与添加聚葡萄糖、菊粉、低聚果糖等膳食纤维相比,抗性糊精能使两种益生菌增殖程度更大,稳定期菌液OD600值达到2.0,表明对抗性糊精的利用率较高。研究结果为水溶性抗性糊精有效调节肠道菌群健康提供了理论支撑。

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    In recent years, a large number of studies have shown that intestinal flora is a key influencing factor of human health, and some dietary fibers such as inulin and polydextrose play an important role in regulating intestinal health. In this paper, starting from the in vitro digestive simulation experiment, we found that the maximum hydrolysis degree of resistant dextrin in simulated gastric fluid and simulated intestinal fluid was 1.3% and 3.7%, respectively, which showed obvious anti-digestive effects, and verified the physiological properties of L P resistant dextrin. The in vitro intestinal probiotic culture study of L P resistant dextrin found that Lactobacillus bulgaricus and Lactobacillus casei were unable to utilize the resistant dextrin as a single nutrient source for their growth, but the addition of a certain amount of resisted dextrin on the basis of a certain nutrient source could promote the proliferation of the two kinds of probiotic bacteria, and the optimal amount of its addition was 0.6% and 0.4% in order. In addition, by comparing different dietary fibers on the growth of probiotics in vitro, it was found that polydextrose, inulin, and oligofructose were able to promote the proliferation of probiotics faster, while resistant dextrin promoted the growth to a greater concentration, and Lactobacillus casei exhibited a higher utilization of resistant dextrin compared to Lactobacillus bulgaricus. Thus, it can be seen that L P resistant dextrin not only has slow digestive properties in vitro, but also effectively promotes the proliferation of beneficial flora in in vitro culture experiments, showing obvious prebiotic effects.

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  • 收稿日期:2024-04-24
  • 最后修改日期:2024-05-09
  • 录用日期:2024-05-15
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