辣木籽蛋白酶解物对小鼠肠道黏膜炎的改善作用

Ameliorating Effects of Moringa oleifera Seed Protein Hydrolysate on Intestinal Mucositis in Mice

摘要:

探讨辣木籽蛋白酶解物（Moringa oleifera Seed Protease Hydrolysate，MSPH）对小鼠肠道黏膜炎的改善作用。提取辣木籽蛋白并进行酶解，冷冻干燥得到MSPH ；随机将雄性BALB/c 小鼠分为空白对照（Con）组、模型（Mod）组和MSPH 组（800 mg/kg•BW），每组6 只，腹腔注射5-FU 诱导小鼠肠道黏膜炎，MSPH 灌胃干预。每天记录小鼠体质量；通过HE 染色观察肠道组织形态学并计算绒毛高度/ 隐窝深度；酶联免疫法测定小鼠血清（INF-γ、SIgA、ROS、G-CSF、ET、D-LA）和小肠组织（IL-1β、IL-6、MDA、MPO、TNF-α）含量变化；实时荧光定量聚合酶链式反应检测小肠组织ZO-1 和Occludin mRNA 表达量。与Mod 组相比，MSPH 组小鼠在实验后期体质量显著提高（P=0.001 1），脾脏和胸腺指数分别增加16.22% 和34.69%，肠绒毛高度/ 隐窝深度增加25.88%，血清中INF-γ、ROS、G-CSF、ET、D-LA 含量分别降低16.85%、17.50%、14.22%、8.28%、9.27%，SIgA 含量升高23.07%，小肠组织中IL-1β、IL-6、MDA、MPO、TNF-α 含量分别降低9.72%、11.23%、9.74%、7.29%、5.17%，肠道组织ZO-1 和Occludin mRNA 表达量增加121.64% 和29.35%。因此，MSPH 可以通过减轻炎症和氧化应激及降低肠道通透性改善5-FU 诱导的小鼠肠道黏膜炎。

Abstract:

The ameliorating effects of Moringa oleifera seed protein hydrolysate (MSPH) on intestinal mucositis in mice were investigated. Moringa oleifera seed protein was extracted, enzymolized, and freeze-dried to obtain MSPH. Male BALB/c mice were randomly divided into the blank control (Con) group, the model (Mod) group, and the MSPH treatment group with six mice in each group. 5-fluorouracil (5-FU) was intraperitoneally injected to induce intestinal mucositis in mice, and MSPH (800 mg/kg•BW) was administered orally for intervention. The weights of the mice were recorded daily. The intestinal histopathology was observed using HE staining, and the villus height/crypt depth was calculated. The concentrations of INF-γ, SIgA, ROS, G-CSF, ET, and D-LA in serum and those of IL-1β, IL-6, MDA, MPO, and TNF-α in the small intestine were determined using enzyme-linked immunosorbent assay. The mRNA expressions of ZO-1 and occluding in the small intestine were determined using real-time fluorescence quantitative polymerase chain reaction. Compared to the mice in the Mod group, the body masses of those in the MSPH group significantly increased (P=0.001 1) in the later stage of the experiment, while their spleen and thymus indices increased by 16.22% and 34.69%, respectively. The villus height/crypt depth increased by 25.88% in the MSPH group. The INF-γ, ROS, G-CSF, ET, and D-LA contents decreased by 16.85%, 17.50%, 14.22%, 8.28%, and 9.27%, respectively, while the SIgA content increased by 23.07% in the MSPH group compared to that in the Mod group. The contents of IL-1β, IL-6, MDA, MPO, and TNF-α decreased by 9.72%, 11.23%, 9.74%, 7.29%, and 5.17%, respectively, while mRNA expressions of ZO-1 and occludin in intestinal tissues increased by 121.64% and 29.35%, respectively, in the MSPH group. Thus, 5-FU-induced intestinal mucositis in mice can be ameliorated by MSPH intervention owing to reduction in inflammation, oxidative stress and intestinal permeability.