富含OPO、OPL和LPL的结构脂质促进C57BL/6小鼠脂质代谢

Structural Lipid Rich in OPO, OPL and LPL Promotes Lipid Metabolism in C57BL/6 Mice

摘要:

该研究通过酶法合成一种富含1,3-二油酸-2-棕榈酸甘油三酯（OPO）、1-油酸-2-棕榈酸-3-亚油酸甘油三酯（OPL）和1,3-二亚油酸-2-棕榈酸甘油三酯（LPL）结构脂质（SL），并研究其对小鼠脂质代谢的影响。选取21只健康的C57BL/6J型实验小鼠均分为3组，喂食无脂纯合日粮，并以灌胃形式分别给予小鼠相同剂量的大豆油，InFat CC（一种富含OPO的产品）和SL（富含OPO、OPL和LPL）。喂养期间观察小鼠体重和摄食量的变化，并检测小鼠血清和肝脏中相关的脂质指标。实验表明，喂养富含OPO、OPL和LPL结构脂质（SL）的小鼠平均体重（24.97 g）低于大豆油（27.29 g）和InFat CC组（26.54 g）小鼠，SL组小鼠肝脏中的总胆固醇TC（0.05 mmol/g prot）、总甘油三酯TG（0.14 mmol/g prot）和脂肪酸合成酶（FAS）质量分数（0.13 mg/g肝脏）与大豆油和InFatCC组相比显著降低（P<0.05）。该研究表明SL能够通过促进肝脏脂质代谢来控制小鼠体重增加，为结构脂质的膳食应用提供理论依据。

Abstract:

In this study, a structured lipid (SL) rich in OPO (1,3-dioleic acid-2-palmitic acid triglyceride), OPL (1-oleic acid-2-palmitic acid-3-linoleic acid triglyceride) and LPL (1,3-dilinoleic acid-2-palmitic triglyceride) was enzymatically synthesized, and the effect of the SL on lipid metabolism in mice was investigated. Twenty-one healthy C57BL/6J experimental mice were selected and divided into 3 groups: a fat-free homozygous diet, with the same dose of soybean oil, InFat CC (an OPO-rich product) or SL (rich in OPO, OPL, and LPL) by gavage. During the feeding period, changes in body weight and food intake were measured, and the related lipid indicators in the serum and liver of the mice were determined. The experimental results showed that the average body weight (24.97 g) of the mice fed with SL was lower than that of the mice in the soybean oil (27.29 g) and InFat CC (26.54 g) groups. The levels of total cholesterol (TC, 0.05 mmol/g protein), total triglyceride (TG, 0.14 mmol/g protein), and fatty acid synthase (FAS) mass fraction (0.13 mg/g liver) in the liver of mice from the SL group were significantly lower than those in the soybean oil group and InFat CC group (P<0.05). The study suggests that SL can control weight gain in mice by promoting hepatic lipid metabolism, thus provids a theoretical basis for the dietary application of structured lipids.