黑豆蛋白和乳清蛋白分别是两种优质的植物源和动物源蛋白质原料，食用后均可改善机体的免疫特性，但双蛋白对机体免疫调节作用的研究相对较少。该试验利用标准膳食（CP）、乳清蛋白膳食（WP）、黑豆蛋白膳食（BSP）和黑豆-乳清双蛋白膳食（BS-WP）对4组大鼠进行为期60 d的喂养，喂养周期结束后对各组大鼠的血清生化指标、免疫球蛋白（Immunoglobulin，Ig）IgM、IgA和IgG质量浓度、脾淋巴细胞增殖指数、自然杀伤细胞活力、脾脏指数和胸腺指数进行测定，探究黑豆乳清双蛋白对大鼠体内免疫的调节作用。结果表明，与单一蛋白源相比，BS-WP组大鼠的血清免疫生化指标总蛋白、白蛋白、球蛋白、尿素氮、和谷丙转氨酶显著提升（P<0.05）；IgG、IgA和IgM的质量浓度均有显著增加（P<0.05），且IgG增加最为显著，与单一WP组和BSP组相比，BS-WP组大鼠的IgG分别增加了38.23%和41.02%，此外，脾脏和胸腺指数也均有显著增加（P<0.05），脾脏指数分别增加了35.33%和35.91%，胸腺指数分别增加了31.51%和34.26%。由此可知，黑豆-乳清双蛋白膳食可显著提升机体免疫能力。

Black soybean protein and whey protein are two kinds of high-quality plant or animal protein materials. Their consumption can improve the immune characteristics of the body. However, there are relatively few studies on the immunomodulatory effect of the black soybean-whey protein blend. In this study, four groups of rats were fed with a standard diet (CP), whey protein diet (WP), black bean protein diet (BSP) and black soybean-whey protein blend diet (BS-WP) for 60 days. At the end of the feeding cycle, serum biochemical indicators, IgM, IgA and IgG mass concentrations, spleen lymphocyte proliferation index, natural killer cell activity, spleen index and thymus index were measured to explore the regulatory effect of black soybean-whey protein blend on the immune system of rats. Results showed that compared with the single protein source group, the serum immune biochemical indexes (total protein, albumin, globulin, urea nitrogen, alanine transaminase levels) of the black soybean-whey protein blend group significantly increased (P<0.05). The mass concentrations of IgG, IgA and IgM increased significantly (P<0.05), with the increase of IgG content being the most significant. Compared with the groups treated whey protein or black soybean protein singly, the IgG content of the protein blend group increased by 38.23% and 41.02%, respectively. In addition, the spleen and thymus indexes significantly increased (P<0.05), with the increase of the spleen index being 35.33% and 35.91%, respectively, and the increase of the thymus index being 31.51% and 34.26%, respectively. Therefore, black soybean-whey blended protein blend diet can significantly improve the body immunity capacities of rats.

国家重点研发计划项目（2018YEE0206300）；黑龙江省博士后面上资助项目（LBH-Z20205）；黑龙江八一农垦大学“三纵”基础培育计划（ZRCPY202004）