基于UPLC-MS/MS分析红豆杉鲜叶和茶叶非挥发性差异成分

Analysis of the Non-volatile Differential Compounds of Fresh Leaves and Tea in Taxus by Using UPLC-MS/MS

摘要:

该研究以曼地亚红豆杉鲜叶（T. media Fresh Leaves，TFL）为材料，通过杀青、揉捻、干燥制成红豆杉茶叶（T. media Tea，TT）。运用超高效液相色谱串联质谱（UPLC-MS/MS）分析比较曼地亚红豆杉鲜叶加工前后的差异化合物。在TFL和TT中共鉴定到796种化合物，TFL中有777种化合物，TT中有768种化合物。根据PCA结合OPLS-DA筛选出差异化合物330种，其中氨基酸、脂质、黄酮、酚酸等255种化合物在TFL中含量高，有机酸等105种化合物在TT中含量高。TFL和TT的关键差异化合物有11种，包括柠康酸、3,5-二羟基-3-甲基戊酸、(2'S3'R)-5-(N,N二甲基-3'-苯基异丝氨酰)-红豆杉三烯A、1,3,5-苯三酚、澳大利亚紫杉碱、3-羟基吡啶、吡咯-2-羧酸在TT中含量较高；苯甲醛、橙皮素-7-O-葡萄糖苷、9-过氧-10E,12Z-十八碳二烯酸、木犀草素-7-O-(6''-咖啡酰)鼠李糖苷在TFL中含量较高。该研究将为红豆杉鲜叶的开发利用提供理论依据。

Abstract:

In this study, Taxus media fresh leaves (TFL) were processed to Taxus media tea (TT) by fixing, rolling and drying, in order to compare the differential compounds between fresh leaves and tea. A total of 796 compounds were identified both in TFL and TT, with 777 compounds in TFL and 768 compounds in TT. Further, according to the analysis PCA and OPLS-DA, 330 differential compounds were identified, of which 255 compounds such as amino acids, lipids, flavonoids, and phenolic acids were significantly reduced in TT, and 105 compounds such as organic acids were significantly increased in TT. Eleven key differential compounds between TFL and TT were further selected, some of which like citraconic acid, mevalonic acid, (2'S,3'R)-5-(N,N-dimethyl-3'-phenylisosery)-taxachitriene A, 1,3,5-benzenetriol,austrotaxine, 3-hydroxypyridine and pyrrole-2-carboxylic acid were higher in TT. Others like benzaldehyde, hesperetin-7-O-glucoside, 9-Oxo-10E,12Z-octadecadienoic acid, and luteolin-7-O-(6''-caffeoyl) rhamnoside were higher in TFL. This study would provide a theoretical basis for the development and utilization of fresh leaves of Taxus.