该文评价了黑果枸杞花青素提取物（Anthocyanins extract of Lycium ruthenicum，AEL）的急性毒性和亚急性毒性。采用固定剂量法一次灌胃AEL 8 000 mg/kg进行经口急性毒性试验，观察小鼠毒性反应，记录14 d内一般状态。选取SD大鼠进行30 d喂养试验，记录大鼠体质量和体征变化，实验结束时进行尿液生化指标、血液学指标、血清生化指标检查，并观察脏器形态变化，计算脏体系数和脏脑系数。研究发现：急性毒性试验中，小鼠无不良反应，体质量正常增长，解剖脏器未见明显异常；连续灌胃30 d后，各组大鼠体质量正常增长，未见明显中毒反应；与对照组比较，高剂量组雌性大鼠单核细胞百分比（M%）显著升高（P<0.05），AST/ALT的比值显著降低（P<0.05）；高剂量组雄性大鼠血清Cl-、脾脏系数高于对照组（P<0.05）。实验组大鼠其余尿液生化指标、血液学指标、血清生化指标、脏体系数和脏脑系数无明显异常。结果表明：小鼠对AEL的单次灌胃最大耐受剂量不低于8 000 mg/kg；连续灌胃30 d，大鼠未见明显中毒反应，AEL在该实验条件下无急性、亚急性毒性，具有较高的安全性。

The acute toxicity and subacute toxicity of an anthocyanins extract of Lycium ruthenicum (AEL) were evaluated. Acute oral toxicity test was performed in mice via intragastric administration of AEL 8 000 mg/kg once by a fixed dose method, and the toxicity responses of the mice were observed while their general states within 14 days were recorded. SD rats were selected for a 30-day feeding experiment, the changes of body weight and physical signs of rats were recorded during theadministration. At the end of the experiment, the biochemical indexes of urine, hematological indexes, and serum biochemical indexes were examined, the morphological changes in organs were observed, and the visceral body coefficient and visceral brain coefficient were calculated. The study revealed that in the acute toxicity test, no side effects was observed in mice, and their body weights increased normally with no obvious abnormalities detected in the anatomical organs. After continuous intragastric administration for 30 days, the rats in each experimental group showed no obvious toxic reaction, and their weights increased normally; Compared with the control group, the percentage of monocytes (M%) in the female high-dose group increased significantly (P<0.05) while their AST/ALT ratios decreased significantly (P<0.05); The serum Cl- and spleen coefficients of the male high-dose group were higher than those of the control group (P<0.05). The other urine biochemical indexes, hematological indexes, serum biochemical indexes, visceral coefficient and visceral brain coefficient of the rats in the experimental group did not show significant abnormalities. The results showed that the maximum tolerable dose for single intragastric administration of AEL to mice was not lower than 8 000 mg/kg, and no toxicity reaction was found in the rats after continuous intragastric administration for 30 days. AEL had no acute and subacute toxicities under the experimental conditions, exhibiting relatively high safety.

国家自然基金面上项目（82174052）；青海省科技基础条件平台建设项目（2020-ZJ-T02）