Abstract:As a science derived from proteomics, peptidomics focuses mainly on the peptides contained in various samples, and is one of the hot topics in the field of food-derived peptide analysis. In recent years, peptidomic analysis technologies have developed rapidly and played an important role in the preparation, analysis, purification, identification and quality control of food-derived active peptides. Moreover, the technologies provide theoretical and technical support at the molecular level for revealing the regulation mechanism of enzymatic hydrolysis of food-derived proteins and the release characteristics of polypeptides. In this paper, the research progress of peptidomic analysis technologies in sample pretreatment, chromatographic separation, mass spectrum detection, structure identification and bioactivity evaluation was reviewed. A brief introduction of their applications in food-derived peptide sequence analysis, guided preparation, quality control, bioactivity prediction and mining was included, in order to provide research direction and theoretical reference for the developments of peptidomics technology and its further application in food research. In the future, peptidomics-based research on the release and action mechanisms of bioactive peptides in food will significantly propel the development of polypeptide industry.
