为优化外源因子胁迫芸豆富集γ-氨基丁酸（γ-Aminobutyric Acid，GABA）的工艺条件，在单因素试验的基础上，利用响应面法探究外源因子添加对萌发芸豆中GABA含量的影响，同时初步对芸豆中GABA积累的机理进行探讨。结果表明，除氯化钠外，谷氨酸钠、氯化钙、抗坏血酸和磷酸吡哆醛均对芸豆萌发富集GABA有显著促进作用；在盐胁迫（3.00 g/L谷氨酸钠、5.37 mmol/L氯化钙）协同0.17 g/L抗坏血酸处理下，萌发24 h的芸豆GABA含量最高为303.60 mg/100 g，是芸豆籽粒和去离子水萌发芸豆的4.39倍和2.12倍；萌发过程中，芸豆GABA积累量与谷氨酸脱羧酶（Glutamic Acid Decarboxylase，GAD）活性呈正相关，底物水平以及Ca2+和H+浓度的提高对胁迫处理促进芸豆积累GABA有积极作用。该研究利用盐胁迫协同抗坏血酸处理有效提高了萌发芸豆中GABA含量，可为开发富含GABA的芸豆食品提供理论参考。

To optimize the process conditions for enriching γ-aminobutyric acid (GABA) in kidney bean under the stress of exogenous factors, the effects of the addition of exogenous factors on the GABA content in germinated kidney bean were investigated by response surface methodology on basis of the single factor experiments. Meanwhile, the mechanism of GABA accumulation in kidney bean was also discussed. The results showed that except for sodium chloride, sodium glutamate, calcium chloride, ascorbic acid and pyridoxal phosphate all significantly promoted GABA enrichment in kidney bean during germination. Under salt stress (3.00 g/L sodium glutamate, 5.37 mmol/L calcium chloride) combined with the treatment with 0.17 g/L ascorbic acid, the GABA content after germination for 24 h was the highest (303.60 mg/100 g), which was 4.39 times and 2.12 times the GABA contents in the kidney bean seeds and in the control kidney bean treated by deionized water, respectively. During germination, the amount of accumulated GABA in kidney bean was positively correlated with the activity of glutamic acid decarboxylase (GAD), and the increase of substrate level and the concentrations of Ca2+ and H+ had positive effects on GABA accumulation in kidney bean under stress treatment. In this research, salt stress combined with ascorbic acid treatment increased effectively the GABA content in germinated kidney bean, which could provide theoretical support for the development of GABA-rich kidney bean products.

企业横向项目（J2020-227）