该文研究了长茎葡萄蕨藻水提物（CLE）体外抗氧化作用，并考察其减轻H2O2诱导的人肝细胞L02氧化损伤的能力。测其成分与含量，通过超氧阴离子和羟自由基来评价CLE的体外抗氧化能力；采用H2O2诱导建立L02细胞氧化损伤模型，以CLE进行干预，CCK8法检测细胞存活率，试剂盒检测胞外乳酸脱氢酶（LDH）、谷草转氨酶（AST）和谷丙转氨酶（ALT）活力以及胞内还原型谷胱甘肽（GSH）和丙二醛（MDA）含量，荧光探针检测细胞内活性氧（ROS）的变化。CLE中总多糖、总三萜、总多酚和总黄酮含量分别为205.01、28.32、27.02和15.72 mg/g，CLE对超氧阴离子和羟自由基均具有较好的清除作用，其半抑制率浓度（IC50）分别为0.94 mg/mL和2.20 mg/mL。与模型组相比，CLE（22.5 μg/mL和45.0 μg/mL）干预提高了L02细胞的存活率，并降低了细胞上清中LDH、AST和ALT活力以及细胞内ROS水平和MDA含量，提高了GSH含量；其中当添加45.0 μg/mL的CLE后，细胞存活率显著提高了20.09%，ROS和MDA含量分别降低至模型组的63.23%和63.20%，GSH含量提高了164.02%。由结果可知，CLE具有较强的体外抗氧化活性，可改善L02细胞氧化损伤，发挥细胞保护作用。

To evaluate the antioxidant activity of water extracts of Caulerpa lentillifera (CLE), and to investigate their ability to reduce H2O2-induced oxidative damage in human L02 cells, the composition and content of CLE was measured, and the in vitro antioxidant effects of CLE were evaluated using superoxide anion and hydroxyl radical scavenging experiments. An oxidative damage model of L02 cells was established by H2O2 induction, and the cell counting kit-8 method was used to determine the survival rate after CLE treatment. The activities of extracellular lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) and the concentrations of intracellular malondialdehyde (MDA) and reduced glutathione (GSH) were determined using commercial kits. Fluorescent probes were used to detect changes in the intracellular reactive oxygen species (ROS) levels. The concentrations of total polysaccharides, total triterpenes, total polyphenols, and total flavonoids in radical scavenging abilities, with half-maximal inhibitory concentrations of 0.94 mg/mL and 2.20 mg/mL, respectively. Compared with the model group, the CLE-treated groups (at 22.5 μg/mL and 45.0 μg/mL) demonstrated higher L02 cell survival rate, reduced LDH, AST, and ALT activities in the cell supernatant, lower intracellular ROS levels and MDA concentration, and increased GSH concentration. With the addition of 45.0 μg/mL of CLE, the cell survival rate was significantly increased by 20.09%, whereas the ROS levels and MDA concentrations decreased to 63.23% and 63.20%, respectively, of their concentrations in the model group. Meanwhile, the GSH concentration increased by 164.02%. These results suggest that CLE have strong antioxidant activity in vitro, and possess the ability to mitigate oxidative damage of L02 cells and provide cytoprotective effects.

国家自然科学基金青年基金项目（31902418）；海南省自然科学基金高层次人才项目（322RC590）；海南大学科研启动基金项目（KYQD（ZR）19108）