为提高青春双歧杆菌存活率及抵抗不利环境的能力，以青春双歧杆菌为研究对象，采用海藻酸钠与乳清蛋白为复合壁材，通过内源乳化法制备微胶囊，并对其进行模拟人工胃肠试验和贮藏稳定性试验。以包埋率为考察指标，根据单因素试验探究海藻酸钠添加量、水相油相体积比、吐温80添加量、搅拌速度和乳化时间等因素对包埋率的影响。采用响应面分析法对海藻酸钠添加量、吐温80添加量、搅拌速度进行分析和优化。结果表明: 制备青春双歧杆菌微胶囊的最佳工艺条件为海藻酸钠添加量2%（m/m），吐温80添加量0.6 mL，搅拌速度430 r/min，包埋率为83.05%。青春双歧杆菌微胶囊于模拟人工胃、肠液中放置180 min，菌体存活率分别为59.89%、66.45%；于4 ℃环境下贮藏42 d，存活率为56.83%。研究表明：以内源乳化法制得的青春双歧杆菌微胶囊对模拟胃肠液环境具有良好的耐酸性及肠溶性，在4 ℃环境中具有良好的贮藏稳定性。

To improve the survival rate of Bifidobacterium adolescentis and its resistance to unfavorable environments, Bifidobacterium adolescentis microcapsules were constructed via endogenous emulsification with sodium alginate and whey proteins as composite wall materials. Simulated artificial gastrointestinal tests and storage stability tests were performed, and, using efficiency as an evaluation index, the effects of sodium alginate addition, the water-to-oil volume ratio, Tween 80 addition, stirring speed, and emulsification time were investigated based on a single factor design. Analysis and optimization of sodium alginate addition, Tween 80 concentration, and stirring speed were performed using response surface methodology; the optimal conditions for the preparation of Bifidobacterium adolescentis microcapsules were 2% sodium alginate, 0.6 mL Tween 80, and a stirring speed of 430 r/min, under which an encapsulation rate of 83.05% was obtained. Bifidobacterium adolescentis microcapsules were then placed in simulated artificial gastric and intestinal juices for 180 minutes to investigate bacterial survival, under which rates of 59.89% and 66.45% were obtained, respectively. Storage was then investigated, and optimum results of 56.83 % survival were obtained for microcapsules retained at 4 ℃ over 42 days. The results demonstrated good acid resistance and enteric solubility for Bifidobacterium adolescentis microcapsules produced by endogenous emulsification and indicated that capsules are stable when stored at 4 ℃.

黑龙江省第一批“揭榜挂帅”科技攻关项目（2021ZXJ03B03）；黑龙江省“百千万”工程科技重大专项（2020ZX07B01-3-2）