该研究利用秀丽隐杆线虫（Caenorhabditis elegans，C. elegans）皮肤损伤模型，研究了罗非鱼皮抗氧化肽（Tilapia Skin Antioxidant Peptide，TSAP）单独或联合维生素C（Vitamin C，Vc）饲喂线虫对其伤口愈合、生存率、体内活性氧（ROS）含量、丙二醛（MDA）含量、超氧化物歧化酶（SOD）以及过氧化氢酶（CAT）的影响，并利用分子对接技术解析TSAP与Vc的协同作用机理。结果表明，与皮肤损伤模型组相比，TSAP单独或联合Vc均能显著提高线虫的伤口愈合率和生存率（P<0.05）。其中，TSAP与Vc联合饲喂的线虫在伤口损伤6 h后的愈合率（21.84%）和损伤后144 h的生存率（43.33%）显著高于TSAP或Vc单独饲喂组（其中TSAP组的伤口愈合率为14.11%，生存率为20.00%；Vc组的伤口愈合率为14.40%，生存率为20.00%，P<0.05）。与皮肤损伤模型组、TSAP组和Vc组相比，TSAP与Vc联合饲喂线虫的细胞内ROS（34.28%）和MDA含量（44.47%）显著降低（P<0.05），SOD（45.24%）和CAT活力（106.57%）显著增加（P<0.05）；分子对接结果表明，TSAP与Vc通过Gly3、Arg8与烯二醇的氢键作用形成复合物，对线虫损伤愈合产生协同作用。综上所述，TSAP和Vc联合饲喂可调控线虫的氧化应激水平，协同促进其伤口愈合。

Caenorhabditis elegans (C. elegans) was used to investigate the impact of combining tilapia skin antioxidant peptide (TSAP) with vitamin C (Vc) for healing skin injuries. The survival rate, reactive oxygen species (ROS) content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and catalase (CAT) activity of C. elegans were determined and the synergistic healing mechanism of TSAP and Vc was analyzed by molecular docking. The healing and survival rates of C. elegans in the treated groups (either TSAP alone or TSAP and Vc) were significantly higher than those in the control (P<0.05). The healing rate after 6 hours (21.84%) and survival rate after 144 hours (43.33%) obtained for C. elegans fed with TSAP and Vc were significantly higher than those of C. elegans fed with TSAP or Vc alone (healing rate of 14.11% and survival rate of 20.00% for TSAP-treated nematodes, P<0.05). Compared with the control, the intracellular ROS (34.28%) and MDA contents (44.47%) of C. elegans fed with TSAP and Vc decreased significantly (P<0.05), whereas the SOD (45.24%) and CAT activities (106.57%) significantly increased (P<0.05). Molecular docking analysis revealed that hydrogen bonds between Gly3, Arg8, and enediol enhance the synergistic healing effect. Conclusively, it is revealed that combining TSAP with Vc has a synergistic effect in terms of oxidative stress level regulation and healing activity in C. elegans.

中央引导地方科技发展资金项目（桂科ZY21195013）